Paternity testing father is absent
DOI:
https://doi.org/10.11606/issn.2317-2770.v5i1-2p6-11Keywords:
Paternity. Polymerase chain reaction/methods. DNA / genetics. Minisatellite. Repeats / genetics. Blotting, southern / methods.Abstract
The aim of this paper is to clarify the professionals involved in Paternity Testing on the different DNA assays currently available (Southern blot or PCR),and focusing specially on the choice of other relatives that should be analyzed if the alleged father is absent. PCR is less cumbersome, allows better reproductibility among results from different laboratories and is indicated when DNA from the alleged father is avaiable. On the other hand, this technique analyzes loci with fewer alleles and may lead to inconclusive results when the DNA from the alleged father cannot be obtained. In these cases, Southern blot should be utilized because in this technique more polymorphic chromossom regions are analyzed. In addition, analysis of the highest possible number of alleged uncles/aunts and alleged brothers/sisters, preferentially envolving also their mothers, is highly recommended.
Downloads
Downloads
Published
Issue
Section
License
Autores que publicam nesta revista concordam com os seguintes termos:
- Autores mantém os direitos autorais e concedem à revista o direito de primeira publicação, que permite o compartilhamento do trabalho com reconhecimento da autoria e publicação inicial nesta revista.
- Autores têm autorização para assumir contratos adicionais separadamente, para distribuição não-exclusiva da versão do trabalho publicada nesta revista (ex.: publicar em repositório institucional ou como capítulo de livro), com reconhecimento de autoria e publicação inicial nesta revista.