Nitrate reductase activity in the diatom Biddulphia longicruris: characterization and daily oscillation

Authors

  • Cristina S Asano Universidade de São Paulo; Instituto de Química
  • Pio Colepicolo Universidade de São Paulo; Instituto de Química
  • Elizabeth Aidar Universidade de São Paulo; Instituto Oceanográfico

DOI:

https://doi.org/10.1590/S1679-87591995000200004

Keywords:

Biddulphia longicruris, Daily variations, Nitrate reductase, Nitrate uptake

Abstract

Nitrate reductase (NR) activity was studied in the marine diatom Biddulphia longicruris. During 24 hours of sampling, NR activity was found during day time and in the transition day-night. Nitrite anions, the product of nitrate reduction, was released by the cells at the times NR was active, and accumulated in the culture medium. Whenever the cultures of B. longicmris were submitted to nitrogen deprivation, NR activity could not be detected. In vitro determination of KM values for NR using nitrate or NADH were respectively 50//M and 80 /*M. Temperature and pH dependence of NR activity were also determined for this organism.

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Published

1995-01-01

Issue

Vol. 43 No. 2 (1995)

Section

Artigos

How to Cite

Nitrate reductase activity in the diatom Biddulphia longicruris: characterization and daily oscillation . (1995). Boletim Do Instituto Oceanografico, 43(2), 123-128. https://doi.org/10.1590/S1679-87591995000200004