Standardization of method for determining glycosylated hemoglobin (Hb A1c) by cation exchange high performance liquid chromatography

Authors

  • Marina Venzon Antunes Instituto de Ciências da Saúde; Centro Universitário Feevale
  • Sandrine Comparsi Wagner Instituto de Ciências da Saúde; Centro Universitário Feevale
  • Joíza Lins Camargo Hospital de Clínicas de Porto Alegre
  • Rafael Linden Instituto de Ciências da Saúde; Centro Universitário Feevale

DOI:

https://doi.org/10.1590/S1984-82502009000400007

Keywords:

High performance liquid chromatography, Mono S^i1^smet, Hemoglobin^i1^s, Hemoglobin^i1^svaria

Abstract

Hemoblobin A1c is the most important parameter for the monitoring of metabolic control of patients with diabetes mellitus. The purpose of this study was to adapt the Mono S method to a conventional HPLC system, allowing highly selective HbA1c determination without the acquisition of kits or the use of dedicated systems The results obtained were compared to the Tinaquant® immune turbidimetric method and the Bio-Rad Variant® chromatographic method. The developed method presented intra-study precision (C.V. %) of 1.39-3.65 and inter-study precision (C.V. %) of 2.80-3.02%. The determination coefficients among methods were: HPLC Mono S x Tinaquant®: r²: 0.9856 (n=60) and HPLC Mono S x HPLC Bio-Rad Variant®: r²: 0.9806 (n=16). A conversion equation between HPLC Mono S and Bio-Rad Variant® was calculated allowing yielding comparable and interchangeable values. The HPLC Mono-S is a precise, low-cost method which yields similar values to the Bio-Rad Variant® method on conventional HPLC equipment.

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Published

2009-12-01

Issue

Section

Original Papers

How to Cite

Standardization of method for determining glycosylated hemoglobin (Hb A1c) by cation exchange high performance liquid chromatography . (2009). Brazilian Journal of Pharmaceutical Sciences, 45(4), 650-657. https://doi.org/10.1590/S1984-82502009000400007