Impact of thyroid hormones and grape juice on biochemical markers and metabolic control proteins in experimental pulmonary arterial hypertension

Isabel Cristina Teixeira  Proença; Patrick  Türck; Vanessa  Duarte Ortiz; Cristina Campos  Carraro; Adriane  Belló-Klein; Alexandre  Luz de Castro; Caroline  Dani; Alex Sander da  Rosa Araujo

doi:10.1590/

Authors

Isabel Cristina Teixeira Proença Laboratory of Cardiovascular Physiology, Federal University of Rio Grande do Sul, UFRGS, Rio Grande do Sul, Brazil https://orcid.org/0000-0002-2560-1975
Patrick Türck Laboratory of Cardiovascular Physiology, Federal University of Rio Grande do Sul, UFRGS, Rio Grande do Sul, Brazil https://orcid.org/0000-0001-5994-5218
Vanessa Duarte Ortiz Laboratory of Cardiovascular Physiology, Federal University of Rio Grande do Sul, UFRGS, Rio Grande do Sul, Brazil https://orcid.org/0000-0002-0420-1569
Cristina Campos Carraro Laboratory of Cardiovascular Physiology, Federal University of Rio Grande do Sul, UFRGS, Rio Grande do Sul, Brazil https://orcid.org/0000-0002-3428-4369
Adriane Belló-Klein Laboratory of Cardiovascular Physiology, Federal University of Rio Grande do Sul, UFRGS, Rio Grande do Sul, Brazil https://orcid.org/0000-0002-8340-7759
Alexandre Luz de Castro Laboratory of Cardiovascular Physiology, Federal University of Rio Grande do Sul, UFRGS, Rio Grande do Sul, Brazil https://orcid.org/0000-0002-4212-8374
Caroline Dani Postgraduate Programme Pharmacology and Therapeutics, Federal University of Rio Grande do Sul, UFRGS, Rio Grande do Sul, Brazil https://orcid.org/0000-0003-0046-7148
Alex Sander da Rosa Araujo Laboratory of Cardiovascular Physiology, Federal University of Rio Grande do Sul, UFRGS, Rio Grande do Sul, Brazil https://orcid.org/0000-0002-9482-7388

DOI:

https://doi.org/10.1590/

Keywords:

PGC-1-alpha, Pyruvate dehydrogenase, Monocrotaline, Food intake

Abstract

Background-Pulmonary arterial hypertension (PAH) increases afterload in the right ventricle (RV), inducing adverse ventricular remodelling. Objective- This study explored the protective effects of thyroid hormones and grape juice on serum biomarkers and proteins related to intermediary metabolism in the RV in a model of PAH. Methods- PAH was induced in Wistar rats via the administration of monocrotaline (60 mg/kg i.p.) and they were subsequently treated with organic grape juice (GJ) and thyroid hormones (TH), administered separately or in combination. Results: The RV systolic diameter significantly increased (20%) in the PAH group compared to the control group (P = 0.002). Total CK, LDH, and ALT levels were reduced (~50%) (P<0.001) in the PAH+TH+GJ group as compared to PAH group. Glucose, albumin, triglyceride, and total cholesterol levels were reduced (~50%) (P<0.001) in the PAH group; however, these parameters returned to baseline in the PAH+GJ group. Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein expression increased two-fold (P<0.001) and PDH protein levels were reduced (~25%) in the PAH+TH+GJ group compared to the PAH group (P<0.001). Conclusion: The data suggest that serum biomarkers can help in the evaluation of this disease, and that this therapeutic approach can attenuate maladaptive remodelling in the PAH model.

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References

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Impact of thyroid hormones and grape juice on biochemical markers and metabolic control proteins in experimental pulmonary arterial hypertension

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