Pathological, molecular and phylogenic study of fowlpox virus in domesticated chickens of Tikrit City, Iraq
DOI:
https://doi.org/10.11606/issn.1678-4456.bjvras.2021.176255Keywords:
FPV, Skin, Nested PCR, Histopathology, FPV 167Abstract
Fowlpox virus (FPV) is one of the viruses affecting chickens worldwide, causing pathological and economic losses in the poultry industry. Viral lesions are easily recognizable by the eye and usually appear in the featherless areas, especially the head. Moreover, the virus could lead to blindness and mortality in some cases. This study diagnosed the suspected fowlpox cases, identified and classified the causative agent. We also analyzed the differences and similarities of closely related viruses at the neighboring and regional countries. Fifty samples were collected from three locations of Tikrit city from the domesticated chickens, which showed cutaneous lesions. Virus DNA was extracted directly from tissue samples before the nested PCR technique was performed. The virion core protein (P4b) gene is partially sequenced and analyzed with routine histological sectioning. Results showed that the virus causes pock lesions of dermal hyperplasia and hyperkeratosis. Hyperplasia and congestion of the chorioallantoic membrane were also recorded. The study also showed that the DNA of FPV could be extracted directly from animal tissue without further purification. The sequence analysis showed that the FPV was confirmed in all samples clustered in clade A identical with Iranian and Egyptian isolates. In conclusion, this study approved that the virus belongs to the classical dermal type of poxviruses and the short genetic distances between viruses related to closely neighboring countries. We also concluded that the conservative P4b gene included mutation sites that make this gene practical for diagnosing the virus and phylogenetic analysis.
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Abdallah FM, Hassanin O. Detection and molecular characterization of avipoxviruses isolated from different avian species in Egypt. Virus Genes. 2013;46(1):63-70. https://doi.org/10.1007/s11262-012-0821-y. PMid:22996736.
Afonso CL, Tulman ER, Lu Z, Zsak L, Kutish GF, Rock DL. The genome of fowlpox virus. J Virol. 2000;74(8):3815-31. https://doi.org/10.1128/JVI.74.8.3815-3831.2000. PMid:10729156.
Al-Hyali MH, Al-Zughaibi AB, Abdulkarim S. Cytopathogenicity studied of fowl pox virus by using indirect immunoperoxidase and acridine orange tests. Iraqi J Vet Medi. 2005;29(2):12-20.
Andersson D, Akrap N, Svec D, Godfrey TE, Kubista M, Landberg G, Ståhlberg A. Properties of targeted preamplification in DNA and cDNA quantification. Expert Rev Mol Diagn. 2015;15(8):1085-100. https://doi.org/10.1586/14737159.2015.1057124. PMid:26132215.
Bayati HAMA. Virological Study about Avipox virus in Local Birds of Iraq. Int J Sci Res (Ahmedabad). 2017;6(6):766-9. https://doi.org/10.21275/ART20174307.
Beytut E, Haligür M. Pathological, immunohistochemical, and electron microscopic findings in the respiratory tract and skin of chickens naturally infected with avipoxvirus. Turk J Vet Anim Sci. 2007;31(5):311-7.
Binns MM, Boursnell MEG, Tomley FM, Campbell J. Analysis of the fowlpoxvirus gene encoding the 4b core polypeptide and demonstration that it possesses efficient promoter sequences. Virology. 1989;170(1):288-91. https://doi.org/10.1016/0042-6822(89)90380-2. PMid:2541544.
Carulei O, Douglass N, Williamson AL. Comparative analysis of avian poxvirus genomes, including a novel poxvirus from lesser flamingos (Phoenicopterus minor), highlights the lack of conservation of the central region. BMC Genomics. 2017;18(1):1-13. https://doi.org/10.1186/s12864-017-4315-0. PMid:29207949.
Domingo E. Virus as Populations. 2nd ed. Academic Press: 2020. Chapter 2 - Molecular basis of genetic variation of viruses: error-prone replication; p. 35-71. https://doi.org/10.1016/b978-0-12-816331-3.00002-7.
Fallavena LCB, Canal CW, Salle CTP, Moraes HLS, Rocha SLS, Pereira RA, Silva AB. Presence of avipoxvirus DNA in avian dermal squamous cell carcinoma. Avian Pathol. 2002;31(3):241-6. https://doi.org/10.1080/03079450220136558. PMid:12396347.
Fenner F, Pereira HG, Porterfield JS, Joklik WK, Downie AW. Family and generic names for viruses approved by the International Committee on Taxonomy of Viruses, June 1974. Intervirology. 1974;3(3):193-8. https://doi.org/10.1159/000149755. PMid:4443189.
Ferreira BC, Ecco R, Couto RM, Coelho HE, Rossi DA, Beletti ME, Silva PL. Outbreak of cutaneous form of avian poxvirus disease in previously pox-vaccinated commercial turkeys. Pesq Vet Bras. 2018;38(3):417-24. https://doi.org/10.1590/1678-5150-pvb-4463.
Gilhare VR, Hirpurkar SD, Kumar A, Naik SK, Sahu T. Pock forming ability of fowl pox virus isolated from layer chicken and its adaptation in chicken embryo fibroblast cell culture. Vet World. 2015;8(3):245-50. https://doi.org/10.14202/vetworld.2015.245-250. PMid:27047081.
Giotis ES, Skinner MA. Spotlight on avian pathology: fowlpox virus. Avian Pathol. 2019;48(2):87-90. https://doi.org/10.1080/03079457.2018.1554893. PMid:30507248.
Jansson L, Hedman J. Challenging the proposed causes of the PCR plateau phase. Biomol Detect Quantif. 2019;17(February):100082. https://doi.org/10.1016/j.bdq.2019.100082. PMid:30886826.
Jarmin S, Manvell R, Gough RE, Laidlaw SM, Skinner MA. Avipoxvirus phylogenetics: identification of a PCR length polymorphism that discriminates between the two major clades. J Gen Virol. 2006;87(8):2191-201. https://doi.org/10.1099/vir.0.81738-0. PMid:16847115.
Laidlaw SM, Skinner MA. Comparison of the genome sequence of FP9, an attenuated, tissue culture-adapted European strain of Fowlpox virus, with those of virulent American and European viruses. J Gen Virol. 2004;85(2):305-22. https://doi.org/10.1099/vir.0.19568-0. PMid:14769888.
Lebdah M, Ali AM, Ali AA, Hassanin O. Insights into pathological and molecular characterization of avipoxviruses circulating in Egypt. Br Poult Sci. 2019;60(6):666-74. https://doi.org/10.1080/00071668.2019.1639141. PMid:31298555.
Lecis R, Secci F, Antuofermo E, Nuvoli S, Cacciotto C, Pittau M, Alberti A. Detection and characterization of an Avipoxvirus in a Common Buzzard (Buteo buteo) in Italy using a multiple gene approach. J Wildl Dis. 2019;55(1):142-8. https://doi.org/10.7589/2017-10-265. PMid:29953311.
Lee LH, Lee KH. Application of the polymerase chain reaction for the diagnosis of fowl poxvirus infection. J Virol Methods. 1997;63(1–2):113-9. https://doi.org/10.1016/S0166-0934(96)02119-2. PMid:9015281.
Le Loc’h G, Bertagnoli S, Ducatez MF. Time scale evolution of avipoxviruses. Infect Genet Evol. 2015;35:75-81. https://doi.org/10.1016/j.meegid.2015.07.031. PMid:26231721.
Le Loc’H G, Ducatez MF, Camus-Bouclainville C, Guérin JL, Bertagnoli S. Diversity of avipoxviruses in captive-bred Houbara bustard. Vet Res (Faisalabad). 2014;45(1):98. https://doi.org/10.1186/s13567-014-0098-3. PMid:25270742.
Manarolla G, Pisoni G, Sironi G, Rampin T. Molecular biological characterization of avian poxvirus strains isolated from different avian species. Vet Microbiol. 2010;140(1–2):1-8. https://doi.org/10.1016/j.vetmic.2009.07.004. PMid:19646826.
Murer L, Westenhofen M, Kommers GD, Furian TQ, Borges KA, Kunert-Filho HC, Streck AF, Lovato M. Identification and phylogenetic analysis of clade C Avipoxvirus in a fowlpox outbreak in exotic psittacines in southern Brazil. J Vet Diagn Invest. 2018;30(6):946-50. https://doi.org/10.1177/1040638718775146. PMid:30199325.
Offerman K, Carulei O, Gous TA, Douglass N, Williamson AL. Phylogenetic and histological variation in avipoxviruses isolated in South Africa. J Gen Virol. 2013;94(Part 10):2338-51. https://doi.org/10.1099/vir.0.054049-0. PMid:23860490.
Ruiz-martı J, Alexander R, Williams J, Herrera-due A, Escudero C, Aguirre I, Benı L. Prevalence and genetic diversity of avipoxvirus in House Sparrows in Spain. Plos One. 2016;11(12):1-13. https://doi.org/10.1371/journal.pone.0168690
Sharma B, Nashiruddullah N, Ahmed JA, Sharma S, Ahamad DB. Pathology of Avipoxvirus isolates in chicken embryonated eggs. Int J Curr Microbiol Appl Sci. 2019;8(09):422-30. https://doi.org/10.20546/ijcmas.2019.809.051.
Soud A, Ibrahim I, El-moaty A. Antigenic and genomic characterization of local fowlpox virus isolate in 2017. J Appl Anim Res. 2020;5(3):31-9.
Suvarna KS, Layton C, Bancroft JD. Bancroft’s theory and practice of histological techniques. China: Churchill Livingstone; 2013. 654 p.
Tantawi HH, Al Sheikhly S, Hassan FK. Avian pox in buzzard (Accipiter nisus) in Iraq. J Wildl Dis. 1981;17(1):145-6. https://doi.org/10.7589/0090-3558-17.1.145. PMid:6265658.
Yeo G, Wang Y, Chong SM, Humaidi M, Lim XF, Mailepessov D, Chan S, How CB, Lin YN, Huangfu T, Fernandez CJ, Hapuarachchi HC, Yap G. Characterization of Fowlpox virus in chickens and bird-biting mosquitoes: A molecular approach to investigating avipoxvirus transmission. J Gen Virol. 2019;100(5):838-50. https://doi.org/10.1099/jgv.0.001209. PMid:30907721.
Zou Z, Zhang J. Morphological and molecular convergences in mammalian phylogenetics. Nat Commun. 2016;7(1):1-9. https://doi.org/10.1038/ncomms12758. PMid:27585543.
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