Comparison of two laparoscopic guided renal biopsy techniques in horses

Abstract

Renal biopsy is a useful diagnostic technique for the identification to the type of lesion, severity of the disease and its evolution. The high incidence of renal lesions in horses, which usually are a consequence of digestive and respiratory diseases and are frequently associated with endotoxemia, prompted us to study the laparoscopic technique for renal biopsy compair the use of needle and forceps. For the purpose of this study ten helthy horses were randomly chosen and divided into two grups of five animals each: in one group laparoscopic guided renal biops was performed using tru-cut needles and in the other group using 5mm laparoscopic forceps. Procedures were carried out with the animals in standing position. Animals were given a sedative analgesic combination and local anesthetic was infiltrated in two specific points on the left flank. One point was used to introduce the trocar and laparoscopic, the other was used to introduce the forceps or the needle. The techniques were compared as regards safety, intensity of bleeding, time for execution and significance of the sample obtained. In all of the procedures, were possible to inspect the abdominal cavity, and chose the place to collect samples, without acidents or complications. The histological analysis of the sample showed a higher percentage of adequate biopsies in the group where laparoscopic forceps were used that permit us to viable both of them with preference for the forcps use.