Sensitivity and specificity of a competitive enzyme immunoassay in the serodiagnosis of bovine brucellosis

Authors

  • Luis Antonio Mathias Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal, SP
  • Allastair Paul Macmillan Central Veterinary Laboratory, Weybridge, Surrey, UK
  • I. Greiser-Wilke Tierarztliche Hochschule Hannover, lnstitut fur Virologie, Hannover, Alemanha
  • V. Moennig Tierarztliche Hochschule Hannover, lnstitut fur Virologie, Hannover, Alemanha

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.1993.52033

Keywords:

Brucellosis (bovina), Serological diagnosis, Competitive Enzyme Immunoassay, Cattle

Abstract

The purpose of this work was to evaluate the sensitivity and the specificity of a competitive enzyme immunoassay, using as conjugate the monoclonal antibodies BM-38 and BM-40, in the serodiagnosis of bovine brucellosis. Seventy-four sera from culture-positive cattle and 2,118 cattle sera from herds free from brucellosis and negative to the Rose Bengal plate test were examined. The competitive enzyme immunoassay, using any of the two conjugates, was able to reveal the presence of antibodies to Brucella lipopolysaccharide in all of the 74 sera of the infected cattle, resulting in a sensitivity of 100%. The specificity of the test using the conjugate BM-38 was 98.82% and using the conjugate BM-40 was 99.95%. These results indicated that the competitive enzyme immunoassay, mainly when using the conjugate BM-40, consists in a technique very useful in the confirmation of the serological diagnosis of bovine brucellosis.

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Published

1993-12-03

Issue

Vol. 30 (1993): Suplemento 1

Section

PREVENTIVE VETERINARY MEDICINE

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

How to Cite

1.
Mathias LA, Macmillan AP, Greiser-Wilke I, Moennig V. Sensitivity and specificity of a competitive enzyme immunoassay in the serodiagnosis of bovine brucellosis. Braz. J. Vet. Res. Anim. Sci. [Internet]. 1993 Dec. 3 [cited 2024 Dec. 22];30(supl.):205-9. Available from: https://revistas.usp.br/bjvras/article/view/52033