Influence of Enzootic Bovine Leukosis on phagocytic function of circulating leukocytes from animals with persistent lymphocytosis

Authors

  • Milton Ricardo Azedo Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Cristina de Oliveira Massoco Cornell University, College of Veterinary Medicine, Department of Clinical Sciences, Ithaca, NY, USA
  • Maiara Garcia Blagitz Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Bárbara Gabriela Soares Sanches Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Fernando Nogueira de Souza Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Camila Freitas Batista Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Mônica Sakai Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia, São Paulo, SP
  • Luiz Carlos de Sá-Rocha Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia, São Paulo, SP
  • José Roberto Kfoury Junior Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Cirurgia, São Paulo, SP
  • Cláudia Regina Stricagnolo Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Fernando José Benesi Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Alice Maria Melville Paiva Della Libera Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2008.26681

Keywords:

Bovine, Leukocytes, Phagocytosis, Enzootic Bovine Leukosis, Flow Cytometry

Abstract

This study evaluated the phagocytosis of propidium iodide-labeled Staphylococcus aureus (PI-Sa) by circulating leukocytes obtained from five Enzootic Bovine Leukosis (EBL)-negative cows, five naturally EBL-infected, non-lymphocytotic cows, and five EBL-positive cows with persistent lymphocytosis (PL), analyzed by flow cytometry. Among cells obtained from EBL-infected cows, presenting PL, the percentage of leukocytes carrying out phagocytosis (7.20%), was smaller (p=0.047) than that verified among cells obtained from non-infected (12.90%), and from BLV-infected, non-lymphocytotic cows (14.70%). Furthermore, leukocytes obtained from EBL-infected cows, presenting PL, showed smaller phagocytosis intensity (characterized by the intensity of propidium iodide fluorescence) than leukocytes obtained from non-infected and from EBL-infected, non-lymphocytotic, cows (p<0.001). Therefore, results show a decreased phagocytic function among circulating leukocytes obtained from BLV-infected, lymphocytotic cows.

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Published

2008-10-01

Issue

Vol. 45 No. 5 (2008)

Section

UNDEFINIED

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

How to Cite

1.
Azedo MR, Massoco C de O, Blagitz MG, Sanches BGS, Souza FN de, Batista CF, et al. Influence of Enzootic Bovine Leukosis on phagocytic function of circulating leukocytes from animals with persistent lymphocytosis. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2008 Oct. 1 [cited 2025 Apr. 24];45(5):390-7. Available from: https://revistas.usp.br/bjvras/article/view/26681