Ginger extract (Zingiber officinale) has anti-cancer and anti-inflammatory effects on ethionine-induced hepatoma rats

Authors

  • Shafina Hanim Mohd Habib Universiti Kebangsaan Malaysia; Faculty of Medicine; Medical Centre; Department of Biochemistry
  • Suzana Makpol Universiti Kebangsaan Malaysia; Faculty of Medicine; Medical Centre; Department of Biochemistry
  • Noor Aini Abdul Hamid Universiti Kebangsaan Malaysia; Faculty of Medicine; Medical Centre; Department of Biochemistry
  • Srijit Das Universiti Kebangsaan Malaysia; Faculty of Medicine; Medical Centre; Department of Anatomy
  • Wan Zurinah Wan Ngah Universiti Kebangsaan Malaysia; Faculty of Medicine; Medical Centre; Department of Biochemistry
  • Yasmin Anum Mohd Yusof Universiti Kebangsaan Malaysia; Faculty of Medicine; Medical Centre; Department of Biochemistry

DOI:

https://doi.org/10.1590/S1807-59322008000600017

Keywords:

Inflammatory markers, TNF-&#945, , NF&#954, B, Chemopreventive, Ginger

Abstract

OBJECTIVE: To evaluate the effect of ginger extract on the expression of NFκB and TNF-α in liver cancer-induced rats. METHODS: Male Wistar rats were randomly divided into 5 groups based on diet: i) control (given normal rat chow), ii) olive oil, iii) ginger extract (100mg/kg body weight), iv) choline-deficient diet + 0.1% ethionine to induce liver cancer and v) choline-deficient diet + ginger extract (100mg/kg body weight). Tissue samples obtained at eight weeks were fixed with formalin and embedded in paraffin wax, followed by immunohistochemistry staining for NFκB and TNF-α. RESULTS: The expression of NFκB was detected in the choline-deficient diet group, with 88.3 ± 1.83% of samples showing positive staining, while in the choline-deficient diet supplemented with ginger group, the expression of NFκB was significantly reduced, to 32.35 ± 1.34% (p<0.05). In the choline-deficient diet group, 83.3 ± 4.52% of samples showed positive staining of TNF-α, which was significantly reduced to 7.94 ± 1.32% (p<0.05) when treated with ginger. There was a significant correlation demonstrated between NFκB and TNF-α in the choline-deficient diet group but not in the choline-deficient diet treated with ginger extract group. CONCLUSION: In conclusion, ginger extract significantly reduced the elevated expression of NFκB and TNF-α in rats with liver cancer. Ginger may act as an anti-cancer and anti-inflammatory agent by inactivating NFκB through the suppression of the pro-inflammatory TNF-α.

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Published

2008-01-01

Issue

Section

Basic Research

How to Cite

Habib, S. H. M., Makpol, S., Hamid, N. A. A., Das, S., Ngah, W. Z. W., & Yusof, Y. A. M. (2008). Ginger extract (Zingiber officinale) has anti-cancer and anti-inflammatory effects on ethionine-induced hepatoma rats . Clinics, 63(6), 807-813. https://doi.org/10.1590/S1807-59322008000600017