Histological study of fresh versus frozen semitendinous muscle tendon allografts
DOI:
https://doi.org/10.1590/S1807-59322010000300010Keywords:
Cryopreservation, Tissue banks, Histology, Tendons, Tissue transplantationAbstract
OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at -80°C in comparison to a control group kept at only -4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 semitendinous tendons from 10 cadavers were frozen at -80ºC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still "fresh". RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous muscle tendon allografts can be submitted to cryopreservation at -80ºC without suffering histological modifications.Downloads
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Published
2010-01-01
Issue
Section
Basic Research
How to Cite
Bitar, A. C., Santos, L. A. U., Croci, A. T., Pereira, J. A. R. M., França Bisneto, E. N., Giovani, A. M. M., & Oliveira, C. R. G. C. M. (2010). Histological study of fresh versus frozen semitendinous muscle tendon allografts . Clinics, 65(3), 297-303. https://doi.org/10.1590/S1807-59322010000300010