Extracellular matrix metalloproteinase expression in endometrial tissue after arterial embolization of myomas

Authors

  • Franco Loeb Chazan Universidade Federal de São Paulo. Departamento de Ginecologia
  • Tatiana C. S. Bonetti Universidade Federal de São Paulo. Departamento de Ginecologia
  • Mariano Tamura Vieira Gomes Universidade Federal de São Paulo. Departamento de Ginecologia
  • Vinicius Adami Vayego Fornazari Universidade Federal de São Paulo. Departamento de Diagnostico por Imagem
  • Manoel João Batista Castello Girão Universidade Federal de São Paulo. Departamento de Ginecologia
  • Claudio Emilio Bonduki Universidade Federal de São Paulo. Departamento de Ginecologia

DOI:

https://doi.org/10.6061/clinics/2021/e2145

Keywords:

Leiomyoma, Extracellular Matrix, Matrix Metalloproteinase, Arterial Embolization of Myomas

Abstract

OBJECTIVES: Arterial embolization of myomas (AEM) is controversial because of the changes that occur in the extracellular matrix (ECM) of the endometrium and its effect on gestational success in infertile patients desiring reproductive capability. Therefore, we performed this study on the expression of genes in the ECM of the endometrium, such as those coding metalloproteinases (MMP), before and 6 months after embolization of the uterine arteries. METHODS: Seven women with leiomyomas were evaluated, and MMP3 and MMP10 levels were measured. The women underwent pelvic nuclear magnetic resonance (NMR), examination, and endometrial biopsy between the 20th and 24th day of the menstrual cycle, and pre- and post-AEM (after 6 months). For data analysis, the Cq comparative method, also known as the 2-DDCT method, was used to calculate the relative quantities of MMP gene expression among the samples collected. RESULTS: There was a significant decrease by 9.52 times in the expression of MMP3 (p=0.007), and a nonsignificant change in the expression of MMP10 (p=0.22) in post-AEM-treated women than pre-AEM-treated women. CONCLUSIONS: The results suggest that ECM continues to undergo tissue remodeling 6 months after AEM, at least with regard to MMP3 expression, suggesting that AEM affects the ECM for at least 6 months after the procedure.

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Published

2021-11-09

Issue

Section

Original Articles

How to Cite

Chazan, F. L. ., Bonetti, T. C. S. ., Gomes, M. T. V. ., Fornazari, V. A. V. ., Girão, M. J. B. C. ., & Bonduki, C. E. . (2021). Extracellular matrix metalloproteinase expression in endometrial tissue after arterial embolization of myomas. Clinics, 76, e2145. https://doi.org/10.6061/clinics/2021/e2145