Effects of ovariectomy and resistance training on oxidative stress markers in the rat liver

Authors

  • Maria Fernanda Cury Rodrigues Federal University of Sao Carlos; Department of Physiological Sciences; Laboratory Exercise of Physiology
  • Uliana Sbeguen Stotzer Federal University of Sao Carlos; Department of Physiological Sciences; Laboratory Exercise of Physiology
  • Mateus Moraes Domingos Federal University of Sao Carlos; Department of Physiological Sciences; Laboratory Exercise of Physiology
  • Rafael Deminice Universidade de Sao Paulo; Faculdade de Medicina de Ribeirao Preto; Laboratory of Nutrition and Metabolism
  • Gilberto Eiji Shiguemoto Federal University of Sao Carlos; Department of Physiological Sciences; Laboratory Exercise of Physiology
  • Luciane Magri Tomaz Federal University of Sao Carlos; Department of Physiological Sciences; Laboratory Exercise of Physiology
  • Nuno Manuel Frade de Sousa Federal University of Sao Carlos; Department of Physiological Sciences; Laboratory Exercise of Physiology
  • Fabiano Candido Ferreira Federal University of Sao Carlos; Department of Physiological Sciences; Laboratory Exercise of Physiology
  • Richard Diego Leite University of Rio de Janeiro; Laboratory Biovasc
  • Heloisa Sobreiro Selistre-de-Araujo Federal University of Sao Carlos; Department of Physiological Sciences; Laboratory Exercise of Physiology
  • Alceu Afonso Jordao-Junior Universidade de Sao Paulo; Faculdade de Medicina de Ribeirao Preto; Laboratory of Nutrition and Metabolism
  • Vilmar Baldissera Federal University of Sao Carlos; Department of Physiological Sciences; Laboratory Exercise of Physiology
  • Sergio Eduardo de Andrade Perez Federal University of Sao Carlos; Department of Physiological Sciences; Laboratory Exercise of Physiology

DOI:

https://doi.org/10.1590/clin.v68i9.76996

Abstract

OBJECTIVE: The objective of this study was to assess the effects of resistance training on oxidative stress markers in the livers of ovariectomized rats. METHOD: Adult Sprague-Dawley rats were divided into the following four groups (n = 8 per group): sham-operated sedentary, ovariectomized sedentary, sham-operated resistance training, and ovariectomized resistance training. During the resistance training period, the animals climbed a 1.1-m vertical ladder with weights attached to their tails; the sessions were conducted 3 times per week, with 4-9 climbs and 8-12 dynamic movements per climb. The oxidative stress was assessed by measuring the levels of reduced glutathione and oxidized glutathione, the enzymatic activity of catalase and superoxide dismutase, lipid peroxidation, vitamin E concentrations, and the gene expression of glutathione peroxidase. RESULTS: The results showed significant reductions in the reduced glutathione/oxidized glutathione ratio (4.11±0.65 nmol/g tec), vitamin E concentration (55.36±11.11 nmol/g), and gene expression of glutathione peroxidase (0.49±0.16 arbitrary units) in the livers of ovariectomized rats compared with the livers of unovariectomized animals (5.71±0.71 nmol/g tec, 100.14±10.99 nmol/g, and 1.09±0.54 arbitrary units, respectively). Moreover, resistance training for 10 weeks was not able to reduce the oxidative stress in the livers of ovariectomized rats and induced negative changes in the hepatic anti-oxidative/oxidative balance. CONCLUSION: Our findings indicate that the resistance training program used in this study was not able to attenuate the hepatic oxidative damage caused by ovariectomy and increased the hepatic oxidative stress.

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Published

2013-09-01

Issue

Section

Basic Research

How to Cite

Rodrigues, M. F. C., Stotzer, U. S., Domingos, M. M., Deminice, R., Shiguemoto, G. E., Tomaz, L. M., Sousa, N. M. F. de, Ferreira, F. C., Leite, R. D., Selistre-de-Araujo, H. S., Jordao-Junior, A. A., Baldissera, V., & Perez, S. E. de A. (2013). Effects of ovariectomy and resistance training on oxidative stress markers in the rat liver. Clinics, 68(9), 1247-1254. https://doi.org/10.1590/clin.v68i9.76996