Methods of biological maceration in the preparation of bat skulls: benefits and limitations
DOI:
https://doi.org/10.11606/1807-0205/2018.58.44Keywords:
Osteologic material, Cleaning, Dermestides, Fly larvae, ChiropteraAbstract
Osteologic collections are used as an aid to scientific and teaching activities, to provide information on specific vertebrate characteristics, such as body support, posture, mode of locomotion, feeding habits and ecological niche. Known techniques for cleaning entire skeletons or mammalian skulls vary according to the destination of the material, the objective of the study, the taxonomic group, and the size of the specimen. The objective of this study was to test three different methods of maceration for cleaning bat skulls (traditional biological maceration, controlled biological maceration using Dermestes maculatus and controlled biological maceration using necrophagous fly larvae). The methods were carried out during five consecutive months, and the minimum and maximum times taken by each method were recorded and documented. Cleaning by fly larvae was the least costly method; the whole process was carried out during the preparation of the osteologic material. The flies ovulated directly on the pieces and the time spent was relatively short. Cleaning by dermestid larvae also resulted in clean parts in a short period, however, its culture needs specific maintenance conditions, since the size of the colony oscillates according to environmental temperature.
Downloads
References
Auricchio, P. & Salomão, M.G. 2002. Técnicas de coleta e preparação de vertebrados para fins didáticos e científicos. Arujá, SP, O Instituto Pau Brasil de História Natural.
Avila-Pires, F.D. 2011. Mudanças nas práticas de coleta e estudo dos mamíferos a partir do século XVIII. Filosofia e História da Biologia, 6(2): 211‑226.
Beaman, R.; Wieczorek J. & Blum, S. 2004. Determining space from place for natural history collections. D‑lib Magazine, 10(5): 9.
Genoways, H.H.; Jones C. & Rossolimo, O.L. 1987. Mammal Collection Management. Lubbock, Tx, Texas Tech University Press.
Hafner, D.J.; Hafner, J.C. & Hafner, M.S. 1984. Skin‐Plus‐Skeleton Preparation as the Standard Mammalian Museum: Specimen. Curator The Museum Journal, 27(2): 141‑145.
Hensel, R.F. 1872. Beiträge zur Kenntniss der Säugethiere Süd-Brasiliens. Berlin, Königlichen Akademie der Wissenschaften. 130p.
INMET – Instituto Nacional de Meteorologia. 2015. Ministério da Agricultura, Pecuária e Abastecimento. Rio de Janeiro, temperatura. Available at: http://www.inmet.gov.br/portal/index.php?r=tempo/graficos. Access in: 17/01/2016.
Köb, E.L. 2006. Ciclo de vida de Dermestes maculatus DeGeer, 1774 (Coleoptera, Dermestidae). Curitiba, Universidade Federal do Paraná. (Monografia).
Marcolino, Z.L. 2014. Dermestes maculatus DeGeer (Coleoptera, Dermestidae) associado a carcaças expostas de Sus scrofa L. em uma área situada em microrregião do sertão paraibano. Campina Grande, Universidade Estadual da Paraíba. (Monografia)
Nunes, D.P. & Perônico, C. 2003. Implantação e proposta de informatização da coleção osteológica de referência do laboratório de zoologia e anatomia comparada do Unileste, MG. Available at: http://www.unilestemg.br/revistaonline/volumes/02/downloads/artigo_19.pdf. Access in: 30/06/2015.
Oliveira, J.A.D.; Bonvicino C.R. & D’Andrea, P.S. 2014. Técnicas de preparação de mamíferos para estudos epidemiológicos: taxidermia, fixação e registro de informações. In: Lemos, E.R.S. & D’Andrea, P.S. (Eds.). Trabalho de campo com animais: procedimentos, riscos e biossegurança. Rio de janeiro, FIOCRUZ. p. 127‑135.
Oliveira-Costa, J.; Dias G.S. & Meloni, E. 2011. Metodologia de coleta para pesquisas experimentais. In: Oliveira-Costa, J. Entomologia Forense: quando os insetos são vestígios. 3.ed. Campinas, SP, Millennium Editora. p. 413‑425.
Papavero, N. 1994. Fundamentos práticos de taxonomia zoológica. São Paulo, UNESP.
Rodrigues, A.B.F.; Lima, A.C.Q.; Nogueira, C.H.O.; Bogossian P.M. & Rocha, V.N. 2012. Utilização de coleópteros na preparação de material osteológico. PUBVET, Londrina, 6: 1277‑1282.
Rose, C.L.; Hawks C.A. & Genoways, H.H. 1995. Storage of natural history collections: A preventive conservation approach. New York, Society for the Preservation of Natural History Collections.
Santos, I.B. & Valverde, M.C.C. 2006. Coleções osteológicas: uma contribuição ao ensino da Zoologia na Universidade Estadual de Feira de Santana, Bahia. Sitientibus Série Ciências Biológicas, 6(2): 162‑164.
Silveira, M.J. & Oliveira, E.F. 2008. A importância das coleções osteológicas para o estudo da biodiversidade. SaBios – Revista de Saúde e Biologia, 3: 1‑4.
Simmons, N.B. & Voss, R.S. 2009. Collection, preparation, and fixation of bat specimens and tissues. In: Kunz, T.H. & Parsons, S. (Eds.). Ecological and behavioral methods for the study of bats. Johns Hopkins University Press, Baltimore, Maryland, p. 849‑867.
Sullivan, L.M. 1999. Wildlife Skull Activities. The University of Arizona Cooperative Extension AZ1145. Available at: https://extension.arizona.edu/pubs. Access in: 09/07/2015.
Sullivan, L.M. & Romney, C.P. 1999. Cleaning and Preserving Animal Skulls. The University of Arizona Cooperative Extension AZ1144. Available at: https://extension.arizona.edu/pubs. Access in: 09/07/2015.
Zaher, H. & Young, P.S. 2003. As coleções zoológicas brasileiras: panorama e desafios. Ciência e Cultura, 55(3): 24‑26.
Downloads
Published
Issue
Section
License
Responsibility: The scientific content and the opinions expressed in the manuscript are the sole responsibility of the author(s).
Copyrights: Papéis Avulsos de Zoologia. The journal is licensed under a Creative Commons attribution-type CC-BY.
