Statistical analysis on the cnidome of genus Hydra using Generalized Linear Models

Authors

DOI:

https://doi.org/10.11606/1807-0205/2022.62.023

Keywords:

Argentina, Cnidocysts, Freshwater cnidarians, Shallow lakes

Abstract

In the systematics of cnidarians, the different types of cnidocysts are considered an important taxonomic character. In Hydra, the four types of cnidocysts found in the ectoderm, concentrated in tentacles and their measurements, together with other morphological and reproductive characteristics, are very important for the taxonomy of the species. In this study, we explore in detail the biometric and statistical characteristics of the cnidome of three species of Hydra collected in three different environments for each climate season. A total of 17,378 capsules were measured. We used ANOVA test and Generalized Linear Model to analyze the distribution and differences reflected in each cnidome, considering the factors “individuals”, “season”, “lagoon” and “species”. The results were clear: the cnidome keep specific information that, together with other taxonomic characteristics, allows us to discern between species of different groups. The same happens with cnidome of the same species but from different lagoons or climatic seasons: we observed a variation of parameters for each type of cnidocyst that could differentiate “ecological races”, since these differences are not enough to declare different species.

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References

Acuña, F.H. & Garese, A. 2009. The cnidae of the acrospheres of the sea anemone Corynactis carnea Studer, 1878 (Cnidaria, Corallimorpharia, Corallimorphidae): composition, abundance and biometry. Belgian Journal of Zoology, 139(1): 50-57.

Acuña, F.H. & Zamponi, M.O. 1997. The use of cnidocysts for ecological races identification from sea anemones populations (Anthozoa, Actiniidae). Iheringia, Sére Zoologia, 82: 9-18.

Acuña, F.H.; Excoffon, A.C. & Ricci, L. 2007. Composition, biometry and statistical relationships between the cnidom and body size in the sea anemone Oulactis muscosa (Cnidaria: Actiniaria). Journal of the Marine Biological Association of the United Kingdom U.K., 87(2): 415-419. https://doi.org/10.1017/S0025315407055087.

Acuña, F.H.; Excoffon, A.C.; Zamponi, M.O. & Ricci, L. 2003. Importance of nematocysts in taxonomy of acontiarian sea anemones (Cnidaria, Actiniaria): A statistical comparative study. Zoologischer Anzeiger, 242: 75-81. https://doi.org/10.1078/0044-5231-00088.

Acuña, F.H.; Ricci, L.; Excoffon, A.C. & Zamponi, M.O. 2004. A novel statistical analysis of cnidocysts in Acontiarian sea Anemones (Cnidaria, Actiniaria) using generalized linear models with Gamma errors. Zoologischer Anzeiger, 243(1-2): 47-52. https://doi.org/10.1016/j.jcz.2004.06.002.

Allcock, A.L.; Watts, P.C. & Thorpe, J.P. 1998. Divergence of nematocysts in two colour morphs of the intertidal beadlet anemone Actinia equina. Journal of the Marine Biological Association of the United Kingdom U.K., 78(3): 821-828. https://doi.org/10.1017/S0025315400044805.

Ardelean, A. & Fautin, D.G. 2004. Variability in nematocysts from a single individual of the sea anemone Actinodendron arboreum (Cnidaria: Anthozoa: Actiniaria). Hydrobiologia, 530/531: 189-197.

Bode, H.R. & Flick, K.M. 1976. Distribution and dynamics of nematocyte populations of Hydra attenuata. Journal of Cell Sciences, 21: 15-34.

Bouillon, J. 1985. Essai de classification des hydropolypes hydroméduses (Hydrozoa-Cnidaria). Indo Malayan Zoology, 1: 29-243.

Burnett, A. 1973. Biology of Hydra. New York, Academic Press.

Cordero, E. 1941a. Observaciones sobre algunas especies sudamericanas del género Hydra. II. Hydra y Cordylophora en el Uruguay. Anais da Academia Brasileira de Ciências, 13(3): 173-183.

Cordero, E. 1941b. Observaciones sobre algunas especies sudamericanas del género Hydra. III. (1) Hydra en Venezuela. (2) La acción de Hydra iheringi sobre las larvas de ciertos peces del nordeste de Brasil. Anais da Academia Brasileira de Ciências, 13(3): 195-201.

Cornelius, P.F.S. & Östman, C. 1987. Redescription of Laomedea exigua M. Sars, a hydroid new to Scandinavia, with comments on its nematocysts, life cycle and feeding movements. Zoologica Scripta, 16: 1-8. https://doi.org/10.1111/j.1463-6409.1987.tb00045.x.

David, C.N.; Ozbek S, Adamczyk, P.; Meier, S.; Pauly, B.; Chapman, J. & Holstein, T.W. 2008. Evolution of complex structures: minicollagens shape the cnidarian nematocyst. Trends in Genetics, 24(9): 431-438. https://doi.org/10.1016/j.tig.2008.07.001.

Deserti, M.I. & Zamponi, M.O. 2011. Hydra vulgaris Pallas, 1766, (= Hydra attenuata) (Cnidaria; Hydrozoa) from the Los Padres Lagoon (Buenos Aires province, Argentina). Revista Brasileira de Biociências, 9(4): 82-487. Available: http://www.ufrgs.br/seerbio/ojs/index.php/rbb/article/view/1753.

Deserti, M.I. & Zamponi, M.O. 2012. Biometric and statistical investigations on the cnidome of the genus Hydra (Cnidaria, Hydrozoa). Iheringia, Série Zoologia, 102(3): 298-302. Available: https://www.scielo.br/j/isz/a/GdYyqnsGWwtq765wbgxmxbm/?lang=en&format=pdf.

Deserti, M.I.; Zamponi, M.O. & Escalante, A.H. 2010. Dos tipos del nematocisto holotrico isorriza en Hydra vulgaris Pallas, 1766 (Cnidaria; Hydrozoa) de diferentes aguas continentales. Revista Real Academia Galega de Ciencias, 29: 67-76.

Deserti, M.I.; Zamponi, M.O. & Escalante, A.H. 2011. The genus Hydra from Argentina. I. Hydra vulgaris pedunculata subsp. nov. (Cnidaria, Hydrozoa). Revista Real Academia Galega de Ciencias, Barcelona, 30: 5-14.

Dioni, W. 1968. Hydra (Chlorohydra) plagiodesmica sp. nov., una hidra verde del río Salado, República Argentina (Cnidaria, Hydrozoa). Physis, 28(76): 203-210.

Ewer, R.F. 1947. On the functions and mode of action of nematocysts of Hydra. Proceedings of the Zoological Society of London, (1947): 365-376. https://doi.org/10.1111/j.1096-3642.1947.tb00524.x.

Fautin, D.G. 2009. Structural diversity, systematics, and evolution of cnidae. Toxicon, 54(8): 1054-1064. https://doi.org/10.1016/j.toxicon.2009.02.024.

Garese, A. 2013. Estudio del cnidoma de anémonas de mar (Cnidaria: Actiniaria y Corallimorpharia): composición, abundancia y biometría. Tesis Doctoral. Mar del Plata, Universidad Nacional de Mar del Plata, Ciencias Marinas.

Garese, A.; Carrizo, S. & Acuña, F.H. 2016. Biometry of sea anemone and corallimorpharian cnidae: statistical distribution and suitable tools for analysis. Zoomorphology, 135(4): 395-404. https://doi.org/10.1007/s00435-016-0319-6.

Gelei, J. 1927. Das Ratsel der Nesselzellen. Sitzungsberichte der Gesellschaft Naturforschender Freunde zu Berlin, (1-3):12-16.

Hastie, T.J. & Tibshirani, R.J. 1990. Generalized additive models. Washington, Chapman & Hall.

Hemmrich, G.; Anokhin, B.; Zacharias, H. & Bosch, T.C. 2007. Molecular phylogenetics in Hydra, a classical model in evolutionary developmental biology. Molecular Phylogenetics and Evolution, 44: 281 290. https://doi.org/10.1016/j.ympev.2006.10.031.

Itô, T. & Inoue, K. 1962. Systematic studies on the nematocysts of Cnidaria. I. Nematocysts of Gymnoblastea and Calyptoblastea. Memoires of the Ehime University. IIB, 4: 445-460.

Kanaev, I.I. 1969. Hydra: essays on the biology of freshwater polyps. Moscow, Soviet Academy of Science. (Trad. por E.T Burrows & H.M. Lenhoff).

Kubota, S. 1976. Notes on the nematocysts of Japanese hydroids, I. Journal of the Faculty of Science, Hokkaido University, Serie 6, Zoology, 20: 230-243.

Nelder, J.A. & Wedderburn, R.W. 1972. Generalized linear models. Journal of the Royal Statistical Society, Series A, 135: 370-384. https://doi.org/10.2307/2344614.

Östman, C. 1979a. Two types of nematocysts in Campanulariidae (Cnidaria, Hydrozoa) studied by light and scanning electron microscopy. Zoologica Scripta, 8: 5-12. https://doi.org/10.1111/j.1463-6409.1979.tb00614.x.

Östman, C. 1979b. Nematocysts in the Phialidium medusae of Clytia hemisphaerica (Hydrozoa, Campanulariidae). Zoon, 7: 125-142.

Östman, C. 1982. Nematocysts and taxonomy in Laomedea, Gonothyraea and Obelia (Hydrozoa, Campanulariidae). Zoologica Scripta, 11: 227-241. https://doi.org/10.1111/j.1463-6409.1982.tb00536.x.

Östman, C. 1983. Taxonomy of Scandinavian hydroids (Cnidaria, Campanulariidae): A study based on nematocyst morphology and isoenzymes. Acta Universitatis Uppsaliensis, 672: 1-22.

Östman, C. 1987. New techniques and old problems in hydrozoans systematic. In: Bouillon, J.; Boero, F.; Cigogna, F. & Cornelius, P.F.S. Modern trends in the systematics, ecology and evolution of Hydroids and Hydromedusae. Oxford, Clarendon Press. p. 67-82.

Östman, C. 1988. Nematocysts as taxonomic criteria within the family Campanulariidae, Hydrozoa. In: Hessinger, D. & Lenhoff, H. The biology of Nematocysts. San Diego, Academic Press Inc. p. 501-517.

Östman, C. 2000. A guideline to nematocyst nomenclature and classification, and some notes on the systematic value of nematocysts. Scientia Marina, 64(1): 31-46. https://doi.org/10.3989/scimar.2000.64s131.

Slautterback, D.B. 1967. The cnidoblast-musculoepithelial cell complex in the tentacles of Hydra. Zeitschrift für Zellforschung und Mikroskopische Anatomie, 79: 296-318. https://doi.org/10.1007/BF00369292.

Toppe, O. 1910. Untersuchungen über Bau und Funktion der Nesselzellen der Cnidarier. Teil I. Der feinere Bau der Nesselzellen sowie systematische Beiträge zur Kenntnis des Genus Hydra. Zoolosche Fahrbücher Abteilung Anatomie und Ontogenie der Tiere, 29: 191-280.

Wang, A.; Deng, L.; Lai, J. & Li, J. 2009. A new species of green hydra (Hydrozoa: Hydrida) from China. Zoological Science, 26: 664-668. https://doi.org/10.2108/zsj.26.664.

Watson, J.E. 1985. The genus Eudendrium (Hydrozoa, Hydroida) from Australia. Proceedings of the Royal Society of Victoria, 97(4): 179-221.

Watts, P.C.; Allcock, A.L.; Lynch, S.M. & Thorpe, J.P. 2000. An analysis of the nematocysts of the beadlet anemone Actinia equina and the green sea anemone Actinia prasina. Journal of the Marine Biological Association of the United Kingdom, 80(4): 719-724.

Weill, R. 1934a. Contribution à l’étude des cnidaires et de leurs nématocystes. I. Recherches sur les nématocystes (morphologie, physiologie, développement). Paris, Laboratoire d’Évolution des Êtres Organisés: Les Presses Universitaires de France.

Weill, R. 1934b. Contribution à l’étude des cnidaires et de leurs nématocystes. II. Valeur taxonomique du cnidome. Paris, Laboratoire d’Évolution des Êtres Organisés: Les Presses Universitaires de France.

Zumstein, Z. 1973. Regulation der Nematocytenâ-Produktion bei Hydra attenuata Pall. Wilhelm Roux’ Archiv, 173: 249-318. https://doi.org/10.1007/BF00575836.

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Published

2022-04-01

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Original Article

How to Cite

Deserti, M. I., & Acuña, F. H. (2022). Statistical analysis on the cnidome of genus Hydra using Generalized Linear Models. Papéis Avulsos De Zoologia, 62, e202262023. https://doi.org/10.11606/1807-0205/2022.62.023