Removing biofilm from a endoscopic: evaluation of disinfection methods currently used

Authors

  • Ana Cristina Balsamo Universidade de São Paulo; Hospital Universitário; Serviço de Controle e Prevenção de Infecção Relacionada à Assistência à Saúde
  • Kazuko Uchikawa Graziano Universidade de São Paulo; Escola de Enfermagem; Departamento de Enfermagem Médico-Cirúrgica
  • René Peter Schneider Universidade de São Paulo; Instituto de Ciências Biomédicas; Departamento de Microbiologia Ambiental
  • Manoel Antunes Junior Universidade de São Paulo; Instituto de Ciências Biomédicas; Departamento de Microbiologia Ambiental
  • Rúbia Aparecida Lacerda Universidade de São Paulo; Escola de Enfermagem; Departamento de Enfermagem Médico-Cirúrgica

DOI:

https://doi.org/10.1590/S0080-62342012000700014

Keywords:

Endoscopes, Biofilms, Disinfection, Central Supply, Hospital, Nursing

Abstract

Laboratory experimental study that compared the effectiveness of five methods of disinfection for the removal of biofilm in gastrointestinal endoscopes. New transparent tubes of polytetrafluoroethylene (Teflon®) were used as specimens to simulate the channels of flexible endoscopes. After pre-cleaning the tubes were intentionally contaminated with Pseudomonas aeruginosa and subjected to disinfection methods. As a result, none removed 100% of these biofilms. What else physically removed biofilm was 2% glutaraldehyde in an automatic processor, probably justified by the double clean, since the equipment has this phase at the beginning of your cycle. The method less effective for removing plaque and other debris was the acidic electrolytic water. These results suggest that the cleaning is most striking in the removal of biofilms that disinfection of consecutive since glutaraldehyde disinfectant by machine is more efficient, it is a fastener organic waste.

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Published

2012-10-01

Issue

Section

Original Article

How to Cite

Balsamo, A. C., Graziano, K. U., Schneider, R. P., Antunes Junior, M., & Lacerda, R. A. (2012). Removing biofilm from a endoscopic: evaluation of disinfection methods currently used . Revista Da Escola De Enfermagem Da USP, 46(spe), 91-98. https://doi.org/10.1590/S0080-62342012000700014