Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis

Authors

  • Mariza Monteiro Universidade de São Paulo; Escola Superior de Agricultura Luíz de Queirós; Departamento de Genética
  • Beatriz Appezzato-da-Glória Universidade de São Paulo; Escola Superior de Agricultura Luíz de Queirós; Departamento de Ciências Biológicas
  • Maria José Valarini Instituto de Zootecnia de Nova Odessa
  • Carlos Alberto de Oliveira Universidade de São Paulo; Escola Superior de Agricultura Luíz de Queirós; Departamento de Genética
  • Maria Lucia Carneiro Vieira Universidade de São Paulo; Escola Superior de Agricultura Luíz de Queirós; Departamento de Genética

DOI:

https://doi.org/10.1590/S0103-90162003000400012

Keywords:

plant biotechnology, in vitro culture, morphogenesis, forage legume

Abstract

Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.

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Published

2003-12-01

Issue

Vol. 60 No. 4 (2003)

Section

Genetics and Plant Breeding

License

All content of the journal, except where identified, is licensed under a Creative Common attribution-type BY-NC.

How to Cite

Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis . (2003). Scientia Agricola, 60(4), 683-689. https://doi.org/10.1590/S0103-90162003000400012