Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost

Authors

  • Chen-Chin Chang Tainan University of Technology; Dept. of Home Science
  • Chang-Chai Ng National Taiwan University; Dept. of Horticulture
  • Chung-Yi Wang National Taiwan University; Dept. of Horticulture
  • Yuan-Tay Shyu National Taiwan University; Dept. of Horticulture

DOI:

https://doi.org/10.1590/S0103-90162009000300004

Keywords:

cellulose degrading, enzymes, vegetable wastes

Abstract

Plant wastes present a high cellulose content, which is an ideal organic material for composting. Five strains of thermophiles from processed Brassica waste were isolated, and the hydrolytic activity on various cellulosic biomass substrata and their temperature profiles were determined. 16S rRNA sequencing identified these strains as Thermoactinomyces and Bacillus spp. Maximal cellulase activity corresponded to 2.3 U mL-1 of enzyme. The application of these strains on Brassica rapa residues demonstrates increased total nitrogen content). TA-3, a Thermoactinomycetes sp. strain, performs best among all inoculants, increasing the nitrogen content from 0.74 to 0.91%, and decreasing the carbon content from 15.4 to 12.2%, showing its high efficiency and bioactivity during compositing.

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Published

2009-06-01

Issue

Vol. 66 No. 3 (2009)

Section

Genetics and Plant Breeding

License

All content of the journal, except where identified, is licensed under a Creative Common attribution-type BY-NC.

How to Cite

Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost . (2009). Scientia Agricola, 66(3), 304-308. https://doi.org/10.1590/S0103-90162009000300004