Spectrophotometric and HPLC determination of deflazacort in pharmaceutical dosage forms

Authors

  • Amarilis Scremin Federal University of Santa Catarina; Department of Pharmaceutical Sciences
  • Monika Piazzon Federal University of Santa Catarina; Department of Pharmaceutical Sciences
  • Marcos Antonio Segatto Silva Federal University of Santa Catarina; Department of Pharmaceutical Sciences
  • Gislaine Kuminek Federal University of Santa Catarina; Department of Pharmaceutical Sciences
  • Giane Márcia Correa The Northwest Regional University of Rio Grande do Sul; Institute of Science, Technology and Health; Faculty of Pharmacy; Department of Health Sciences
  • Niraldo Paulino Bandeirante University of São Paulo; Institute of Post-graduation; Group for Research and Development in Phytomedicine
  • Simone Gonçalves Cardoso Federal University of Santa Catarina; Department of Pharmaceutical Sciences

DOI:

https://doi.org/10.1590/S1984-82502010000200015

Keywords:

Deflazacort^i1^squantitative determinat, High performance liquid chromatography^i1^squantitative analy, Spectrophotometry^i1^squantitative analy, Medicines^i1^squantitative analy

Abstract

Deflazacort (DFZ) is a glucocorticoid used as an anti-inflammatory and immunosuppressant drug. No official methods are available for DFZ determination in pharmaceutical formulations. The objective of this study was to develop, validate and compare spectrophotometric (UV and colorimetric) and high-performance liquid chromatography (HPLC) methods, for the quantitative determination of DFZ in tablets and oral suspension. For the UV method, ethanol was used as the solvent, with detection at 244 nm. The colorimetric method was based on the redox reaction with blue tetrazolium in alkaline medium, with detection at 524 nm. The method by HPLC was carried out using a C18 column, mobile phase consisting of acetonitrile:water (80:20, v/v) with a flow rate of 1.0 mL min-1 and detection at 244 nm. The methods proved linear (r > 0.999), precise (RSD < 5%) and accurate (recovery > 97%). Statistical analysis of the results indicated that the UV and HPLC methods were statistically equivalent, while the values obtained for the colorimetric method differed significantly from the other methods.

Downloads

Downloads

Published

2010-06-01

Issue

Vol. 46 No. 2 (2010)

Section

Original Papers

License

All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.

The on-line journal has open and free access.

How to Cite

Spectrophotometric and HPLC determination of deflazacort in pharmaceutical dosage forms . (2010). Brazilian Journal of Pharmaceutical Sciences, 46(2), 281-287. https://doi.org/10.1590/S1984-82502010000200015