Bioactivity evaluation of least explored traditionally acclaimed medicinally potent herb Nanorrhinum ramosissimum(Wall.) Betsche

Authors

  • Jyotsna Sharma Shri Mata Vaishno Devi University. School of Biotechnology, Jammu & Kashmir, India https://orcid.org/0000-0002-9159-5518
  • Savita Sharma Shri Mata Vaishno Devi University. School of Biotechnology, Jammu & Kashmir, India
  • Anuja Koul Shri Mata Vaishno Devi University. School of Biotechnology, Jammu & Kashmir, India
  • Raju Shankarayan Shri Mata Vaishno Devi University. School of Biotechnology, Jammu & Kashmir, India
  • Sharada Mallubhotla Shri Mata Vaishno Devi University. School of Biotechnology, Jammu & Kashmir, India https://orcid.org/0000-0002-0880-5394

DOI:

https://doi.org/10.1590/s2175-97902023e19334

Keywords:

Branched cancerwort, Antimicrobial activity, Antioxidant activity, Alpha amylase inhibition, DPPH assay

Abstract

Present study analysed the therapeutic potential of traditionally acclaimed medicinal herb Nanorrhinum ramosissimum, using plant parts extracted with different solvents (10 mg/mL). Shoot extracts exhibited comparatively better antimicrobial properties, in comparison to root extracts. Total phenolic content was estimated, to ascertain its dependency on antioxidant properties of plant extracts. Antioxidant assay revealed promising results in comparison to IC50 value of standard ascorbic acid (52.2±0.07 µg/mL), for methanolic extracts of shoot (61.07±0.53 µg/mL and 64.33±0.33 µg/mL) and root (76.705±0.12 µg/mL and 89.73±0.28 µg/ mL) for in vivo and in vitro regenerants respectively. Correlation coefficient R2 values ranged between 0.90-0.95, indicating a positive correlation between phenolic contents and antioxidant activity. Plant extracts were also able to inhibit DNA oxidative damage again indicating their antioxidative potential. Antidiabetic potential was confirmed by alpha amylase inhibition assay where shoot methanolic extracts (invivoin vitro) exhibited the best IC50 values (54.42±0.16 µg/mL, 66.09±0.12 µg/mL) in comparison to standard metformin (41.92±0.08 µg/mL). Ethanolic extracts of roots (in vitro, invivo) exhibited the relative IC50 values (88.97±0.32µg/mL,96.63±0.44 µg/mL) indicating that shoot parts had a better alpha amylase inhibition property; thus proving the herb’s bioactive potential and its prospective therapeutic source for curing various ailments.

Downloads

Download data is not yet available.

References

Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat Protoc. 2007;2(4):875-877.

Amin A, Emmy T, Kenn F, Vassiliki E, Sandra A, Luc P, et al. In vitro and in silico antidiabetic and antimicrobial evaluation of constituents from Kickxia ramosissima (Nanorrhinum ramosissimum). Front Pharmacol. 2017;8:232.

Anagnostopoulou MA, Kefalas P, Papageorgiou VP, Assimopoulou AN, Boskou D. Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis). Food Chem. 2006;94(1):19-25.

Anitha Gopal B, Muralikrishna G. Porcine pancreatic α-amylase and its isoforms: Purification and kinetic studies. Int J Food Prop. 2009;12(3):571-86.

Babbar N, Oberoi HS, Uppal DS, Patil RT. Total phenolic content and antioxidant capacity of extracts obtained from six important fruit residues. Food Res Int. 2011;44(1):391-396.

Bajaj S, Khan A. Antioxidants and diabetes. Indian J Endocr Metab. 2012;16(Suppl 2):S267-S271.

Balakrishnan B, Paramasivam S, Arulkumar A. Evaluation of the lemongrass plant (Cymbopogon citratus) extracted in different solvents for antioxidant and antibacterial activity against human pathogens. Asian Pac J Trop Dis. 2014;4(1):S134-S139.

Bhandari C. Vanousadhi Chandrodaya: An Encyclopedia of Indian Botanist and Herbs. Varanasi: Chaukhamba Sanskrut Sansthan; 2006.117p.

Burt S. Essential oils: Their antibacterial properties and potential application in foods: A review. Int J Food Microbiol. 2004;94(3):223-253.

Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem . 2006;97(4):654-660.

Feresin GE, Tapia A, Gutierrez RA, Delporte C, Backhouse EN, Schmeda-Hirschmann G. Free radical scavengers, anti-inflammatory and analgesic activity of Acaena magellanica. J Pharm Pharmacol. 2002;54(6):835-844.

Ghorbani A. Best herbs for managing diabetes: a review of clinical studies. Braz J Pharm Sci. 2013;49(3):413-422.

Gill AO, Holley RA. Disruption of Escherichia coli, Listeria monocytogenes and Lactobacillus sakei cellular membranes by plant oil aromatics. Int J Food Microbiol . 2006;108(1):1-9.

Jan S, Khan MR. Antipyretic, analgesic and antiinflammatory effects of Kickxia ramosissima. J Ethnopharmacol.2016;182:90-100.

Kanatt SR, Chander R, Sharma A. Antioxidant potential of mint (Mentha spicata L.) in radiation-processed lamb meat. Food Chem . 2007;100(2):451-458.

Kim DO, Chun OK, Kim YJ, Moon HY, Lee CY. Quantification of polyphenolics and their antioxidant capacity in fresh plums. J Agric Food Chem.2003;51(22):6509-6515.

Koleva II, Van Beek TA, Linssen JPH, de Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochem Analysis. 2002;13(1):8-17.

Kirtikar KR, Basu BD. Indian medicinal plants. 2nd ed. DehraDun: International Book Distributors; 2005.

Myo EM, Maung CE, Mya KM, Khai AA. Characterization of bacterial endophytes from Myanmar medicinal plants for antimicrobial activity against human and plant pathogens. Braz J Pharm Sci . 2020;56:e17705.

Nanumala SK, Tulasi P, Sujitha E. In vitro anti-diabetic activity of seed extracts of Cassia auriculata and Cassia angustifolia. Eur J Exp Biol. 2015;5(5):12-17.

Pandya PN, Aghera HB, Ashok BK, Acharya R. Diuretic activity of Linaria ramosissima (Wall.) Janch. leaves in albino rats. Ayu. 2012;33(4):576-578.

Piluzza G, Bullitta S. Correlations between phenolic content and antioxidant properties in twenty-four plant species of traditional ethnoveterinary use in the Mediterranean area. Pharm Biol. 2011;49(3):240-247.

Pullaiah T, Naidu K. Antidiabetic plants in India and herbal based antidiabetic research. New Delhi: Regency Publications; 2003.

Sharma J, Koul A, Sharma S, Shankarayan R, Mallubhotla S. In vitro propagation of Nanorrhinum ramosissimum (Wall.) Betsche: A traditionally important medicinal plant. Kuwait JSci. 2021;48(3).

Siti HN, Kamisah Y, Kamsiah J. The role of oxidative stress, antioxidants and vascular inflammation in cardiovascular disease (a review). Vasc Pharmacol. 2015;71:40-56.

Tabart J, Kevers C, Sipel A, Pincemail J, Defraigne JO, Dommes J. Optimisation of extraction of phenolics and antioxidants from black currant leaves and buds and of stability during storage. Food Chem . 2007;105(3):1268-1275.

West IC. Radicals and oxidative stress in diabetes. Diabet Med. 2000;17(3):171-180.

Zhao M, Yang B, Wang J, Li B, Jiang Y. Identification of the major flavonoids from pericarp tissues of lychee fruit in relation to their antioxidant activities. Food Chem . 2006; 98(3):539-544.

Downloads

Published

2023-05-08

Issue

Section

Original Article

How to Cite

Bioactivity evaluation of least explored traditionally acclaimed medicinally potent herb Nanorrhinum ramosissimum(Wall.) Betsche. (2023). Brazilian Journal of Pharmaceutical Sciences, 59, e19334. https://doi.org/10.1590/s2175-97902023e19334