Nephroprotective effects of Lippia sidoides ethanolic extract against ischemia/reperfusion-induced acute kidney injury

Marcus Felipe Bezerra da Costa; Tiago Lima Sampaio; Isabella Evelyn Prado de Azevedo; Dânya Lima Bandeira; Igor Lima Soares; Gdayllon Cavalcante Meneses; Samilly Albuquerque Ribeiro; Janaina Serra Azul Monteiro Evangelista; Mary Anne Medeiros Bandeira; Alexandre Braga Libório; Alice Maria Costa Martins

doi:10.1590/s2175-97902023e21371

Authors

Marcus Felipe Bezerra da Costa Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Ceará, Brazil http://orcid.org/0000-0002-6121-1202
Tiago Lima Sampaio Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, Ceará, Brazil http://orcid.org/0000-0002-3962-6508
Isabella Evelyn Prado de Azevedo Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, Ceará, Brazil http://orcid.org/0000-0002-7047-9091
Dânya Lima Bandeira Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, Ceará, Brazil http://orcid.org/0000-0002-6395-8561
Igor Lima Soares Department of Pharmacy, Federal University of Ceará, Brazil http://orcid.org/0000-0003-0082-2891
Gdayllon Cavalcante Meneses Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, Ceará, Brazil http://orcid.org/0000-0002-0160-5728
Samilly Albuquerque Ribeiro Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Ceará, Brazil
Janaina Serra Azul Monteiro Evangelista School of Veterinary Medicine, State University of Ceará, Fortaleza, Ceará, Brazil http://orcid.org/0000-0002-9583-1573
Mary Anne Medeiros Bandeira Department of Pharmacy, Federal University of Ceará, Brazil http://orcid.org/0000-0003-4301-4739
Alexandre Braga Libório Post-graduate Program, Universidade de Fortaleza-UNIFOR, Fortaleza, Ceará, Brazil http://orcid.org/0000-0002-0409-6113
Alice Maria Costa Martins Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, Ceará, Brazil http://orcid.org/0000-0001-8160-2027

DOI:

https://doi.org/10.1590/s2175-97902023e21371

Keywords:

Alecrim-pimenta, Acute kidney injury, Nephrin, Thymol

Abstract

Ischemia/reperfusion injury (I/R) is commonly related to acute kidney injury (AKI) and oxidative stress. Antioxidant agents are used to treat this condition. Lippia sidoides is a brazillian shrub with anti-inflammatory and anti-oxidative properties. Thus, the aim of this study is to evaluate the effect of Lippia sidoides ethanolic extract (LSEE) on in vivo and in vitro models of AKI induced by I/R. Male Wistar rats were submitted to unilateral nephrectomy and ischemia on contralateral kidney for 60 min via clamping followed by reperfusion for 48 h. They were divided into four groups: Sham, LSEE (sham-operated rats pre-treated with LSEE), I/R (rats submitted to ischemia) and I/R-LSEE (rats treated with LSEE before ischemia). Kidney tissues homogenates were used to determine stress parameters and nephrin expression. Plasma and urine samples were collected for biochemical analysis. I/R in vitro assays were evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and flow cytometry assays in Rhesus Monkey Kidney Epithelial Cells (LLC-MK2). The LSEE treatment prevented biochemical and nephrin expression alterations, as well as oxidative stress parameters. In the in vitro assay, LSEE protected against cell death, reduced the reactive oxygen species and increased mitochondrial transmembrane potential. LSEE showed biotechnological potential for a new phytomedicine as a nephroprotective agent.

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Nephroprotective effects of Lippia sidoides ethanolic extract against ischemia/reperfusion-induced acute kidney injury

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