Authentication of Brazilian Ginseng using Bar-HRM analysis

Francine Alves Nogueira de Almeida; Pablo Viana  Oliveira; Natan Silva Matos; Verônica Luiza Luiza Silveira Fialho; Magda Delorence Lugon; Maria do Carmo  Vieira; Márcia Flores da Silva  Ferreira; Greiciane Gaburro Paneto

doi:10.1590/s2175-97902023e21179

Authors

Francine Alves Nogueira de Almeida Universidade Federal do Espírito Santo, Vitória, ES, Brazil https://orcid.org/0000-0002-1633-9477
Pablo Viana Oliveira Programa de Pós-Graduação em Biotecnologia https://orcid.org/0000-0002-1785-178X
Natan Silva Matos Universidade Federal do Espírito Santo, Alegre, ES, Brazil
Verônica Luiza Luiza Silveira Fialho Universidade Federal do Espírito Santo, Vitória, ES, Brazil
Magda Delorence Lugon Universidade Federal do Espírito Santo, Vitória, ES, Brazil https://orcid.org/0000-0001-8156-7903
Maria do Carmo Vieira Universidade Federal de Grandes Dourados. Faculdade de Ciências Agrárias, MS, Brazil https://orcid.org/0000-0001-7047-3848
Márcia Flores da Silva Ferreira Universidade Federal do Espírito Santo, Alegre, ES, Brazil https://orcid.org/0000-0003-1541-6634
Greiciane Gaburro Paneto Universidade Federal do Espírito Santo. Universidade Federal do Espírito Santo https://orcid.org/0000-0001-8035-4199

DOI:

https://doi.org/10.1590/s2175-97902023e21179

Keywords:

Amaranthaceae;, Eriantha;, Glomerata;, Hebanthe;, Pfaffia.

Abstract

Hebanthe eriantha (Martius) Kuntze and Pfaffia glomerata (Spreng) Pedersen are medicinal plants popularly known as “Brazilian Ginseng” due to their similarity to Panax ginseng. In Brazil, they are sold as the same herb, despite their different pharmacological and toxicological properties. The morphological identification is difficult, which facilitates their adulteration. We report the application of the Barcode DNA High-Resolution Melting (Bar-HRM) using matK gene to differentiate both species in samples sold in the Brazilian market. Using the proposed method, we could discriminate and identify both species. Bar-HRM analysis allowed discriminating and identifying both species. It allowed the identification of H. eriantha and P. glomerata in 43.6% and 56.4% of the amplified samples, respectively. Of these, only seven samples were authenticated and, in 71.4% of the cases, adulterated. We concluded that Bar-HRM has proven to be a fast alternative method to authenticate plants under the common name “Brazilian Ginseng”.

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References

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Authentication of Brazilian Ginseng using Bar-HRM analysis

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