Effect of polycaprolactone nanocapsules loaded with essential oils on biofilm formation by Staphylococcus aureus strains isolated from bovine mastitis cases

Authors

  • Gabriela Suthovski Universidade Federal da Fronteira Sul, Realeza, Paraná, Brazil
  • Alcione Santa Catarina Universidade Federal da Fronteira Sul, Realeza, Paraná, Brazil
  • Diana Paula Perin Universidade Federal da Fronteira Sul, Realeza, Paraná, Brazil
  • Rubiana Mara Mainardes Universidade Estadual do Centro Oeste, Guarapuava, Paraná, Brazil
  • Karina Ramirez Starikoff Universidade Federal da Fronteira Sul, Realeza, Paraná, Brazil
  • André Lazarin Gallina Universidade Estadual do Centro Oeste, Guarapuava, Paraná, Brazil
  • Maiara Garcia Blagitz Azevedo Universidade Federal da Fronteira Sul, Realeza, Paraná, Brazil
  • Fabíola Dalmolin Universidade Federal da Fronteira Sul, Realeza, Paraná, Brazil
  • Luciana Velasques Cervo Universidade Federal da Fronteira Sul, Realeza, Paraná, Brazil https://orcid.org/0000-0001-7611-1135
  • Dalila Moter Benvegnú Universidade Federal da Fronteira Sul, Realeza, Paraná, Brazil https://orcid.org/0000-0002-3419-9674

DOI:

https://doi.org/10.1590/

Keywords:

Nanoencapsulation;, Citronella;, Melaleuca;, Bovine mastitis;, Biofilm;, Bacteria;, Essential oil

Abstract

Bovine infectious mastitis is largely resistant to antibacterial treatment, mainly due to mechanisms of bacterial resistance in the biofilms formed by Staphylococcus aureus. Melaleuca (MEO) and citronella essential oils (CEO) are promising agents for reducing or eliminating biofilms. Free melaleuca oil presented a medium Minimum Inhibitory Concentration (MIC) of 0.625% and a Minimum Bactericidal Concentration (MBC) of 1.250%, while free citronella oil showed medium MIC and MBC of 0.313%. Thus, free CEO and MEO demonstrate bacteriostatic and bactericidal potential. We generated polymeric nanocapsules containing MEO or CEO and evaluated their efficacy at reducing biofilms formed by S. aureus. Glass and polypropylene spheres were used as test surfaces. To compare the responses of free and encapsulated oils, strains were submitted to 10 different procedures, using free and nanoencapsulated essential oils (EOs) in vitro. We observed no biofilm reduction by MEO, free or nanoencapsulated. However, CEO nanocapsules reduced biofilm formation on glass (p=0.03) and showed a tendency to diminish biofilms on polypropylene (p=0.051). Despite nanoencapsulated CEO reducing biofilms in vitro, the formulation could be improved to modify the CEO component polarity and, including MEO, to obtain more interactions with surfaces and the biofilm matrix.

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Published

2023-08-28

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Vol. 59 (2023)

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How to Cite

Effect of polycaprolactone nanocapsules loaded with essential oils on biofilm formation by Staphylococcus aureus strains isolated from bovine mastitis cases. (2023). Brazilian Journal of Pharmaceutical Sciences, 59, e23068. https://doi.org/10.1590/