Interaction between FAK/αB-crystalline is important for viability of the glioblastoma cells

Michelle Bueno de Moura Pereira; João Eustáquio  Antunes; Raquel  Tognon-Ribeiro; Luana  Lulio; Mariany Lopes da Costa  Folly

doi:10.1590/

Authors

Michelle Bueno de Moura Pereira Departamento de Ciências Básicas da Vida, Universidade Federal de Juiz de Fora Campus Governador Valadares (UFJF-GV), MG, Brazil https://orcid.org/0000-0003-2065-1700
João Eustáquio Antunes Departamento de Farmácia, Universidade Federal de Juiz de Fora, Campus Governador Valadares (UFJF-GV), MG, Brazil https://orcid.org/0000-0003-1693-5823
Raquel Tognon-Ribeiro Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas (UNIFAL-MG), MG, Brazil https://orcid.org/0000-0002-4184-706X
Luana Lulio Universidade Federal de Juiz de Fora, Campus Governador Valadares (UFJF-GV), MG, Brazil https://orcid.org/0009-0004-7336-390X
Mariany Lopes da Costa Folly Departamento de Ciências Básicas da Vida, Universidade Federal de Juiz de Fora Campus Governador Valadares (UFJF-GV), MG, Brazil https://orcid.org/0000-0001-9523-6187

DOI:

https://doi.org/10.1590/

Keywords:

Glioblastoma, FAK, αB-crystalline, Peptides, Protein interaction

Abstract

Glioblastoma multiforme is a tumor of the central nervous system. Focal Adhesion Kinase (FAK) and αB-crystalline are two proteins involved in glioblastoma development. In this study, we investigated whether the FAK/αB-crystalline interaction is important for glioblastoma cells, we aimed to investigate the interaction of these two proteins in the glioblastoma multiforme cell line U87-MG. Two peptides named FP01 peptide (derived from αB-crystalline) and FP02 peptide (derived from FAK) were synthesized for this study. Treatment of U87-MG with the peptides FP01 and FP02 in the concentration at 50 µM reduced the viability cellular to around 41% and 51%, respectively. Morphological alterations in the cells treated with the peptides when compared to the control were observed. This study suggests that the interaction between FAK and αB-crystalline is important for the viability of glioblastoma cells.

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Interaction between FAK/αB-crystalline is important for viability of the glioblastoma cells

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