Non-invasive biomagnetic assessment of gastrointestinal motility in a loperamide-induced constipation model

Erick Guilherme   Stoppa; Guilherme Augusto   Soares; Lais Pereira  Buranello; Luiz Marcos Frediani  Portela; Luiz Antônio   Justulin; Madileine Francely  Américo; Luiz Claudio   Di Stasi; José Ricardo de Arruda  Miranda

doi:10.1590/

Authors

Erick Guilherme Stoppa Department of Biophysics and Pharmacology, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, SP, Brazil https://orcid.org/0000-0002-0903-6021
Guilherme Augusto Soares Department of Biophysics and Pharmacology, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, SP, Brazil https://orcid.org/0000-0002-4723-466X
Lais Pereira Buranello Department of Biophysics and Pharmacology, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, SP, Brazil https://orcid.org/0000-0002-7469-3482
Luiz Marcos Frediani Portela Department of Strucutral and Functional Biology, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, SP, Brazil https://orcid.org/0000-0002-5898-3615
Luiz Antônio Justulin Department of Strucutral and Functional Biology, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, SP, Brazil https://orcid.org/0000-0001-6142-3515
Madileine Francely Américo Institute of Biological Sciences and Health, Federal University of Mato Grosso, UFMT, Barra do Garças, Brazil https://orcid.org/0000-0001-8474-3765
Luiz Claudio Di Stasi Department of Biophysics and Pharmacology, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, SP, Brazil https://orcid.org/0000-0002-7864-1073
José Ricardo de Arruda Miranda Department of Biophysics and Pharmacology, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, SP, Brazil https://orcid.org/0000-0002-8306-8056

DOI:

https://doi.org/10.1590/

Keywords:

Alternating Current Biosusceptometry (ACB), Loperamide, Constipation, Gastrointestinal Transit, Colonic Motility

Abstract

Constipation is a disorder of the gastrointestinal (GI) and some of the main etiological mechanisms are directly related to changes in GI physiology. The capacity to carry out paired assessments and measure GI parameters under the influence of constipation is a relevant point in selecting a suitable methodology. We aimed to perform a non-invasive investigation of gastrointestinal motility in constipated rats using the alternating current biosusceptometry system (ACB). The animals were split into two groups: the pre-induction stage (CONTROL) and post-induction loperamide stage (LOP). We assessed GI motility parameters using the ACB system. Colon morphometric and immunohistochemical analyses were performed for biomarkers (C-kit) for interstitial cells of Cajal (ICC). Our results showed a significant increase in gastrointestinal transit in the LOP group in addition to a reduction in the dominant frequency of gastric contraction and an arrhythmic profile. A change in colonic contractility profiles was observed, indicating colonic dysmotility in the LOP group. We found a reduction in the number of biomarkers for intestinal cells of Cajal (ICC) in the LOP group. The ACB system can evaluate transit irregularities and their degrees of severity, while also supporting research into novel, safer, and more efficient treatments for constipation.

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Non-invasive biomagnetic assessment of gastrointestinal motility in a loperamide-induced constipation model

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