Microrna-135A-5P promotes proliferation and metastasis of hepatocelluar carcinoma cells via upregulation of FOXO1

Tian  Zhang; Lingling  Guan; Xiaomeng  Xie; Lixing  Guo; Dongjing  Ni

doi:10.1590/

Authors

Tian Zhang Department of Pathology, Shiyan People’s Hospital of Bao’an District, China https://orcid.org/0009-0003-4091-5918
Lingling Guan Department of Gastroenterology, The Fifth Affiliated Hospital of Jinan University (Shenhe People’s Hospital), China https://orcid.org/0009-0008-9462-5963
Xiaomeng Xie Department of Gastroenterology, The Fifth Affiliated Hospital of Jinan University (Shenhe People’s Hospital), China https://orcid.org/0009-0004-5294-8327
Lixing Guo Department of Oncology, The Fifth Affiliated Hospital of Jinan University (Shenhe People’s Hospital), China https://orcid.org/0009-0009-3211-0662
Dongjing Ni Department of Gastroenterology, The Fifth Affiliated Hospital of Jinan University (Shenhe People’s Hospital), China https://orcid.org/0009-0007-7523-4820

DOI:

https://doi.org/10.1590/

Keywords:

Hepatocellular carcinoma, MicroRNA-135a-5p, Transcription factor forkhead box O1, Proliferation, Metastasis

Abstract

Numerous studies have focused on microRNAs (miRNAs) in hepatocellular carcinoma (HCC). However, the specific role of miR-135a-5p in HCC has remained unclear. Thus, this study aimed to elucidate the role and mechanisms of action of miR-135a-5p in HCC. Transfected Huh-7 HCC cells were subjected to cell proliferation, apoptosis, migration, and invasion assays to investigate the role of miR-135a-5p and FOXO1 in modulating cellular functions. Additionally, tumor xenografts from nude mice were utilized in vivo to validate the in vitro findings. Downregulation of miR-135a-5p or upregulation of FOXO1 inhibited cell proliferation and metastasis of Huh-7 cells while promoting apoptosis. Conversely, upregulation of miR-135a-5p induced proliferation and metastasis of Huh-7 cells while suppressing apoptosis. Furthermore, FOXO1 was identified as a direct target of miR-135a-5p. Notably, downregulation of miR-135a-5p or upregulation of FOXO1 suppressed tumor growth in Huh-7 cells. Inhibition of miR-135a-5p impeded HCC cell proliferation and metastasis by upregulating FOXO1 expression.

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Microrna-135A-5P promotes proliferation and metastasis of hepatocelluar carcinoma cells via upregulation of FOXO1

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