A hydrogel based herbal nanoformulation for enhanced wound healing
DOI:
https://doi.org/10.1590/Keywords:
Nanoparticles, Rhubarb, Design Expert, Wound healingAbstract
The aim of the present study is to develop a chitosan based herbal formulation using rhubarb for wound healing. Wound healing is a dynamic process facilitating the regeneration or repair of broken tissue. Rhubarb is commonly used worldwide for their effective antiseptic, antifungal and antiviral properties. Rhubarb loaded chitosan nanoparticles were prepared by ionic cross-linking process with sodium lauryl sulphate. The optimized formulation was examined for antibacterial activity against S. aureus and P. aeruginosa. Wound healing effects of the developed formulation was studied on excision wound model in Wistar albino rats. Average particle size and zeta potential of chitosan nanoparticles varied from 93 to 2,225 nm and from+30.82 to -16.08 mV, respectively. On the basis of particle size, zeta potential, and % entrapment, Formulation C 4 (0.0595% chitosan, 0.535% cross-linker, and 1% drug) was selected among different checkpoint formulation and further studies were performed. Hydrogel was developed using a combination of polyvinylpyrrolidone and carboxymethyl cellulose. Nanoformulation based hydrogel showed significantly enhanced antimicrobial activity against tested pathogenic microbial strains in comparison to drug solution, as well as marketed formulations.
Downloads
References
Agarwal M, Agarwal MK, Shrivastav N, Pandey S, Das R, Gaur P. Preparation of chitosan nanoparticles and their in-vitro characterization. Int J Life Sci Sci Res. 2015; 1713-1720.
Ahmad SB, Rehman MU, Ahmad SP, Mudasir S, Rahman Mir M. Rheum emodi L. (Rhubarb) promotes wound healing by decreasinginflammatory markers and enhancing accumulation of biomolecules. Ann Phytomed. 2017; 6(2): 114-118.
Ahmed S, Ikram S. Chitosan based scaffolds and their applications in wound healing. Adv Life Sci. 2016; 10(1): 27-37.
Amidi M, Mastrobattista E, Jiskoot W, Hennink WE. Chitosan-based delivery systems for protein therapeutics and antigens. Adv Drug Delivery Rev. 2010; 62: 59-82.
Asasutjarit R, Sorrachaitawatwong C, Tipchuwong N, Pouthai S. Effect of Formulation Compositions on Particle Size and Zeta Potential of Diclofenac Sodium-Loaded Chitosan Nanoparticles. Int J Pharmacol Pharm Sci. 2013; 7(9): 568-570.
Baker BP, Grant JA. Sodium lauryl sulfate profile active ingredient eligible for minimum risk pesticide use. New York State Integrated Pest Management. 2018: 1-12.
Bhattarai N, Gunn J, Zhang M. Chitosan-based hydrogels for controlled, localized drug delivery. Adv Drug Delivery Rev. 2010; 62: 83-99.
Cheung R, Ng T, Wong JH, Chan WY. Chitosan: An update on potential biomedical and pharmaceutical applications. Mar Drugs. 2015; 13(8): 5156-5186.
Chhabra P, Mehra L, Mittal G, Kumar A. A comparative study on the efficacy of chitosan gel formulation and conventional silver sulfadiazine treatment in healing burn wound injury at molecular level. Asian J Pharm. 2017; 11(3): 489.
Dai T, Tanaka M, Huang YY, Hamblin MR. Chitosan preparations for wounds and burns: antimicrobial and wound-healing effects. Expert Rev Anti Infect Ther. 2011; 9(7): 857-879.
Dons T, Soosairaj S. Evaluation of wound healing effect of herbal lotion in albino rats and its antibacterial activities. Int J Phytomedic Phytother. 2018; 4: 6.
Dreifke MB, Jayasuriya AA, Jayasuriya AC. Current wound healing procedures and potential care. Mater Sci Eng C Mater Biol Appl. 2015; 48: 651-662.
Eppley BL, Woodall JE, Higgins J. Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plast Reconstr Surg. 2004; 114(6): 1502-8.
Farahpour MR, Habibi M. Evaluation of the wound healing activity of an ethanolic extract of Ceylon cinnamon in mice. Vet Med. 2012; 57(1): 57-53.
Gowda ASD, Suresh J, Aravind Ram ASA, Srivastava A, Ali MOR. Formulation and evaluation of topical gel using Eupatorium glandulosummichx for wound healing activity. Pharm Lett. 2016; 8(8): 255-266.
Guidance on the operation of the Animals (Scientific Procedures) Act 1986. https://www.gov.uk/guidance/guidance-on-the-operation-of-the-animals-scientific-procedures-act-1986
» https://www.gov.uk/guidance/guidance-on-the-operation-of-the-animals-scientific-procedures-act-1986
Hammond CR. The Elements, in Handbook of Chemistry and Physics 81st edition. CRC press. 2014.
Ikobi E, Igwilo C, Awodele O, Azubuike P. Antibacterial And wound healing properties of methanolic extract of dried fresh Gossypium Barbadense Leaves. AJBPS. 2012; 2(13): 32-37.
Jagtap NS, Khadabadi SS, Farooqui IA, Nalamwar VP, Sawarkar HA. Development and evaluation of herbal wound healing formulations. Int J Pharm Tech Res. 2009; 1(4): 1104-8.
Jana S, Jana S. Antibacterial Activity of Chitosan-Based Systems. Functional Chitosan. 2020; 6: 457-489.
Jang HW, Hsu WH, Hengel MJ, Shibamoto T. Antioxidant activity of Rhubarb (Rheum rhabarbarum L.) extract and its main component emodin. Nat Prod Chem Res. 2018; 6(3): 1-4.
Jayakumar R, Prabaharan M, Sudheesh Kumar PT, Nair SV, Tamura H. Chitosan-based hydrogels for controlled, localized drug delivery. Biotechnol Adv. 2011; 29: 322-337.
Koohsari H, Ghaemi EA, Sheshpoli MS, Jahedi M, Zahiri M. The investigation of antibacterial activity of selected native plants from North of Iran. J Med Life. 2015; 8(2): 38-42.
Liu H, Wanga C, Li C, Qina Y, Wanga Z, Yanga F, et al. A functional chitosan-based hydrogel as a wound dressing and drug delivery system in the treatment of wound healing. RSC Adv. 2018; 8: 7533-7549.
Madhava CK, Sivaji K, Tulasirao K. Flowering plants of Chittor District, Andhra Pradesh. Tirupati, India: Students offset printers. 2008; 277.
Malik MA, Rehman MU, Bhat SA, Ahmad SB. Phytochemical analysis and antimicrobial activity of Rheum emodi (Rhubarb)rhizomes. Pharma Innovation J. 2018; 7(5): 17-20.
Nahar M, Mishra D, Dubey V, Jain NK. Development, characterization, and toxicity evaluation of amphotericin B-loaded gelatin nanoparticles. Nanomed.: Nanotechnol. Biol. Med. 2008; 4(3): 252-261.
Namasivayam SKR, Samydurai S, Chettia T. Nanoformulation of Antibacterial Antibiotics Cefpirome with Biocompatible Polymeric Nanoparticles and Evaluation for the Improved Antibacterial Activity and Nontarget Toxicity Studies. Asian J Pharm. 2017; 11(2): S269-S281.
Patel AM, Kurbetti SM, Savadi RV, Thorat VA, Takale VV, Horkeri SV. Preparation and Evaluation of Wound Healing Activity of New Polyherbal Formulations in Rats. American Journal of Phytomedicine and Clinical Therapeutics. AJPCT. 2013; 1(6): 498-506.
Piret J, Désormeaux A, Cormier H, Lamontagne J, Gourde P, Juhász J, et al. Sodium lauryl sulfate increases the efficacy of a topical formulation of foscarnet against herpes simplex virus type 1 cutaneous lesions in mice. Antimicrob Agents Chemother. 2000; 44(9): 2263-70.
Ryu S, Song PI, Seo CH, Cheong H, Park Y. Colonization and infection of the skin by S. aureus: Immune system evasion and the response to cationic antimicrobial peptides. Int J Mol Sci. 2014; 15(5): 8753-8772.
Sayyahi J, Mobaiyen H, Jafari B, Sales AJ. Antibacterial effects of methanolic extracts of Reumribes L. and Hyssopus officinalis on some standard pathogenic bacteria. JBJ. 2020; 7(3): 35-44.
Sharma D, Maheshwari D, Philip G, Rana R, Bhatia S, Singh M, et al. Formulation and optimization of polymeric nanoparticles for intranasal delivery of lorazepam using Box-Behnken design: in vitro and in vivo evaluation. BioMed Res. Int. 2014.
Shinde U, Ahmed MH, Singh K. Development of dorzolamide loaded 6-O-carboxymethyl chitosan nanoparticles for open angle glaucoma. J Drug Deliv. 2013; 1-15.
Sun SB, Liu P, Shao FM, Miao QL. Formulation and evaluation of PLGA nanoparticles loaded capecitabine for protate cancer. Int J Clin Exp Med. 2015; 8(10): 19670-19681.
Tegos G, Stermitz FR, Lomovskaya O, Lewis K. Multidrug pump inhibitors uncover remarkable activity of plant antimicrobials. Antimicrob Agents Chemother. 2002; 46(10): 3133-41.
Wani TU, Raza SN, Khan NA. Rosmarinic acid loaded chitosan nanoparticles for wound healing. Int J Pharma Sci Res. 2018; 19: 1138-1147.
Wu JR, Lu YC, Hung SJ, Lin JH, Chang KC, Chen JK, et al. Antimicrobial and Immunomodulatory Activity of Herb Extracts Used in Burn Wound Healing: “San Huang Powder”. Evid Based Complement Alternat Med. 2021; 2021: 2900060.
Yien L, Zin NM, Sarwar A, Katas H. Antifungal activity of chitosan nanoparticles and correlation with their physical properties. Int J Biomater. 2012; 632698.
Zubair M, Malik A, Ahmad J. Clinico-microbiological study and antimicrobial drug resistance profile of diabetic foot infections in North India. Foot. 2011; 21: 6-14.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Brazilian Journal of Pharmaceutical Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.
The on-line journal has open and free access.
