Células-tronco derivadas de diferentes tecidos possuem morfologia, proliferação, viabilidade, plasticidade e potencial mitótico semelhantes
DOI:
https://doi.org/10.11606/issn.1678-4456.bjvras.2024.219450Palavras-chave:
Células-tronco mesenquimais derivadas de tecido adiposo, Células estromais, Suínos, SenescênciaResumo
As células-tronco mesenquimais (CTM) são células somáticas multipotentes, amplamente estudadas devido à sua plasticidade e imunomodulação. Estudos sugerem que células-tronco derivadas de diferentes tecidos podem originar populações heterogêneas de células e, ainda, discutem sobre os efeitos negativos de sucessivas passagens sobre a atividade mitótica dessas células. Diante disso, este estudo avaliou as diferenças na morfologia, proliferação, plasticidade e viabilidade de MSCs suínas derivadas de medula óssea (BM-MSCs) e tecido adiposo (ADMSCs) e o impacto da cultura até a 10ª passagem em seu ciclo celular. MSCs derivadas de tecido adiposo suíno e medula óssea foram cultivadas em meio D-MEM suplementado com soro fetal bovino, aminoácidos não essenciais, L-glutamina e penicilina estreptomicina. Essas células foram expandidas em dez passagens, caracterizadas morfologicamente e imunofenotipicamente e avaliadas quanto à viabilidade, proliferação e plasticidade e ciclo celular. Nossos resultados identificaram dois tipos de células, ADSCs e BMMSCs, que exibiram morfologia fusiforme, expressão de CD90+/CD105+/CD14- e características semelhantes de proliferação (ADMSCs: 2,34 ± 1,0x105; BM-MSCs: 2,20 ± 0,62 x105), viabilidade (ADMSCs: 84,6%; BM-MSCs: 80,9%) e plasticidade. Além disso, ambos os grupos de células apresentaram concentração crescente de células em G0/G1 a partir da sexta passagem. Em conclusão, a morfologia, proliferação, viabilidade, plasticidade e imunofenótipo de ADMSCs e BMMSCs suínos são semelhantes. Além disso, é possível sugerir que ambos os grupos de células apresentam redução gradual do potencial mitótico a partir da 6ª passagem.
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