Effect of phytogenic additives on ruminal parameters, in vitro and in situ degradability in beef cattle
DOI:
https://doi.org/10.11606/issn.1678-4456.bjvras.2025.220460Keywords:
Croton urucurana Baillon, Fatty acids short-chain, Monensin, ProtozoaAbstract
Technological advances, easily pursued by research on ruminant nutrition, can improve animal production, prevent nutritional disorders, and increase digestibility concomitantly with reducing ingestive discrepancies. This study aimed to investigate the inclusion of crude extract of Croton urucurana (Croton) or essential oils of cashew and castor bean on in vitro and in situ degradability of Tifton 85 hay and metabolic parameters in beef cattle. The experiment was conducted at the Biological Sciences Institute (ICB) and the Veterinary School of the Federal University of Goias, Brazil. The treatments were: Control, Monensin, Croton, and Blend (essential oil composed of cashew and castor bean). We used five replicates to evaluate ruminal parameters and dry matter degradability. Blend increased dry matter degradation, indicating that forage feeds last longer in the rumen and tend to be more degraded with Blend inclusion. The assessments of ad libitum ingestion of dry matter showed that monensin treatment reduced feed intake compared to control and Croton treatments. Our results suggest that the inclusion of Blend improves the degradability of dry matter in the diet and could be a strategy of choice in diets based on low-quality forage.
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Adeniji YA, Sanni MO, Mutassim AM. Review: manipulation of the rumen using additives. Rep Opin J. 2020;12(2):1-6. http://doi.org/10.7537/marsroj120220.01.
Albornoz RI, Harvatine KJ, Allen MS. Diet starch concentration and starch fermentability affect energy intake and energy balance of cows in the early postpartum period. J Dairy Sci. 2019;102(6):5161-71. http://doi.org/10.3168/jds.2018-15634. PMid:30981484.
Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils: a review. Food Chem Toxicol. 2008;46(2):446-75. http://doi.org/10.1016/j.fct.2007.09.106. PMid:17996351.
Benchaar C, Duynisveld JL, Charmley E. Effects of monensin and increasing dose levels of a mixture of essential oil compounds on intake, digestion and growth performance of beef cattle. Can J Anim Sci. 2006;86(1):91-6.
Bouda J, Quiroz-Rocha GF, Gonzalez FHD. Importância da coleta e análise de líquido ruminal e urina. In: Gonzalez FHD, Borges JB, Cecim M, editors. Uso de provas de campo e laboratório em doenças metabólicas e ruminais de bovinos. Porto Alegre: UFRGS; 2000. p. 13-6.
Chaney AL, Marbach EP. Modified reagents for determination of urea and ammonia. Clin Chem. 1962;8(2):130-2. http://doi.org/10.1093/clinchem/8.2.130. PMid:13878063.
Chen H, Wang C, Huasai S, Chen A. Effects of dietary forage to concentrate ratio on nutrient digestibility, ruminal fermentation and rumen bacterial composition in Angus cows. Sci Rep. 2021;11(1):17023. http://doi.org/10.1038/s41598-021-96580-5. PMid:34426627.
Chilliard Y, Ferlay A, Doreau M. Effect of different types of forages, animal fat or marine oils in cow’s diet on milk fat secretion and composition, especially conjugated linoleic acid (CLA) and polyunsaturated fatty acids. Livest Prod Sci. 2001;70(1):31-48. http://doi.org/10.1016/S0301-6226(01)00196-8.
Dehority BA. Evaluation of subsampling and fixation procedures used for counting rumen protozoa. Appl Environ Microbiol. 1984;48(1):182-5. http://doi.org/10.1128/aem.48.1.182-185.1984. PMid:6476828.
Díaz TG, Branco AF, Ítavo LCV, Santos GT, Carvalho ST, Teodoro AL, Oliveira RL. In vitro gas production kinetics and digestibility in ruminant diets with different levels of cashew nut shell liquid. Semina: Ciênc Agrár. 2018;39(4):1669. http://doi.org/10.5433/1679-0359.2018v39n4p1669.
European Union. Commission notice: guidelines for the prudent use of antimicrobials in veterinary medicine [Internet]. Official Journal of the European Union; Luxembourg; 2015 [cited 14 Mar 2024], nº 299). Available from: https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=CELEX:52015XC0911(01).
Galyean ML, Goetsch AL. Utilization of forage fiber by ruminants. In: Hatfield RD, Buxton DR, Jung HG, Mertens DR, Ralph J, Weimer PJ, editors. Forage cell wall structure and digestibility madison. Madison: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America; 1993. http://doi.org/10.2134/1993.foragecellwall.c2.
Goetz BM, Horst EA, Mayorga EJ, Abeyta MA, Rodriguez- Jimenez S, Carta S, Lourenco JM, Callaway TR, Hikita C, Watanabe T, Baumgard LH. Effects of cashew nut shell extract supplementation on production, rumen fermentation, metabolism, and inflammatory biomarkers in transition dairy cows. J Dairy Sci. 2023;106(12):9843-54. http://doi.org/10.3168/jds.2023-23563. PMid:37641319.
Grummer RR, Mashek DG, Hayirli A. Dry matter intake and energy balance in the transition period. Vet Clin North Am Food Anim Pract. 2004;20(3):447-70. http://doi.org/10.1016/j.cvfa.2004.06.013. PMid:15471620.
Gurgel LA. Estudo das atividades antifúngica, anti- inflamatória intestinal e antinocieptiva visceral do látex do Croton urucurana Baill. [thesis]. Fortaleza: Universidade Federal do Ceará; 2005. Kamra DN, Agarwal N, Chaudhary LC. Inhibition of ruminal methanogenesis by tropical plants containing secondary compounds. Int Congr Ser. 2006;1293:156-63. http://doi.org/10.1016/j.ics.2006.02.002.
Latimer Junior GW, editor. Official methods of analysis of AOAC International. 21st ed. Arlington: AOAC International; 2019. 3390 p. http://doi.org/10.1093/9780197610145.001.0001.
Li MM, Ghimire S, Wenner BA, Kohn RA, Firkins JL, Gill B, Hanigan MD. Effects of acetate, propionate, and pH on volatile fatty acid thermodynamics in continuous cultures of ruminal contents. J Dairy Sci. 2022;105(11):8879-97. http://doi.org/10.3168/jds.2022-22084 PMid:36085109.
Martínez S, Madrid J, Hernández F, Megías MD, Sotomayor JA, Jordán MJ. Effect of thyme essential oils (Thymus hyemalis and Thymus zygis) and monensin on in vitro ruminal degradation and volatile fatty acid production. J Agric Food Chem. 2006;54(18):6598-602. http://doi.org/10.1021/jf060985p PMid:16939315.
McGaw L. Use of plant-derived extracts and essential oils against multidrug-resistant bacteria affecting animal health and production. In: Rai MK, Kon KV, editors. Fighting multidrug resistance with herbal extracts, essential oils and their components. Amsterdam: Academic Press-Elsevier; 2013. p. 191-203. http://doi.org/10.1016/B978-0-12-398539-2.00013-6.
McMeniman JP, Tedeschi LO, Defoor PJ, Galyean ML. Development and evaluation of feeding-period average dry matter intake prediction equations from a commercial feedlot database. J Anim Sci. 2010;88(9):3009-17. http://doi.org/10.2527/jas.2009-2626. PMid:20453082.
Mourthe MHF, Reis RB, Ladeira MM, Souza RC, Coelho SG, Saturnino HM. Multiple supplement with ionophores for grazing steers: intake, ruminal fermentation, and in situ degradability. Arq Bras Med Vet Zootec. 2011;63(1):129-35. http://doi.org/10.1590/S0102-09352011000100020.
Nader TT. Atividade antibacteriana in vitro de extratos e substâncias isoladas de espécies de Croton frente Staphybcoccus aureus causador de mastite bovina. [thesis]. Jaboticabal: Universidade Estadual Paulista; 2014.
Naz R, Bano A. Antimicrobial potential of Ricinus communis leaf extracts in different solvents against pathogenic bacterial and fungal strains. Asian Pac J Trop Biomed. 2012;2(12):944- 7. http://doi.org/10.1016/S2221-1691(13)60004-0. PMid:23593573.
Nocek JE. Evaluation of specific variables affecting in situ estimates of ruminal dry matter and protein digestion. J Anim Sci. 1985;60(5):1347-58. http://doi.org/10.2527/jas1985.6051347x.
Oliveira AP, Reis RA, Bertipaglia LMA, Melo GMP, Berchielli TT, Oliveira JA, Casagrande DR, Balsalobre MAA. Substituição de monensina sódica por bicarbonato de sódio em dietas de novilhas confinadas. Arq Bras Med Vet Zootec. 2013;65(4):1149-57. http://doi.org/10.1590/S0102-09352013000400030.
Oliveira IS, Lima JCS, Silva RM, Martins DTO. Triagem da atividade antibacteriana in vitro do látex e extratos de Croton urucurana Baillon. Rev Bras Farmacogn. 2008;18(4):587-93. http://doi.org/10.1590/S0102-695X2008000400016.
Orskov ER, McDonald I. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J Agric Sci. 1979;92(2):499- 503. http://doi.org/10.1017/S0021859600063048.
Osmari MP, Matos LF, Salab BL, Diaz TG, Giotto FM. Cashew nut shell liquid: characteristics and applicability in animal production. Pubvet. 2015;9(3):143-9. http://doi.org/10.22256/pubvet.v9n3.143-149.
Palma ASV, Barra NC, Herling VR, Gomide CA, Netto AS. Supplementation with nutritional additives and organic minerals on the performance of newly weaned Nellore calves on pasture. Pesq Agropec Bras. 2015;50(11):1071-8. http://doi.org/10.1590/S0100-204X2015001100010.
Park T, Yang C, Yu Z. Specific inhibitors of lysozyme and peptidases inhibit the growth of the rumen protozoan Entodinium caudatum without decreasing feed digestion or fermentation in vitro. J Appl Microbiol. 2019;127(3):670-82. http://doi.org/10.1111/jam.14341. PMid:31165532.
Patra AK. Meta-analyses of effects of phytochemicals on digestibility and rumen fermentation characteristics associated with methanogenesis. J Sci Food Agric. 2010;90(15):2700-8. http://doi.org/10.1002/jsfa.4143. PMid:20740549.
Patra AK. Enteric methane mitigation technologies for ruminant livestock: a synthesis of current research and future directions. Environ Monit Assess. 2012;184(4):1929-52. http://doi.org/10.1007/s10661-011-2090-y. PMid:21547374.
Patra AK, Saxena J. A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen. Phytochemistry. 2010;71(11-12):1198-222. http://doi.org/10.1016/j.phytochem.2010.05.010. PMid:20570294.
Patra AK, Yu Z. Effects of essential oils on methane production and fermentation by, and abundance and diversity of, rumen microbial populations. Appl Environ Microbiol. 2012;78(12):4271-80. http://doi.org/10.1128/AEM.00309-12. PMid:22492451.
R Core Team. R: a language and environment for statistical computing [software]. Vienna: R Foundation for Statistical Computing; 2020 [cited 2022 Oct 12]. Available from: https://www.r-project.org/.
Salem AZM, Kholif AE, Elghandour MMY, Buendía G, Mariezcurrena MD, Hernandez SR, Camacho LM. Influence of oral administration of Salix babylonica extract on milk production and composition in dairy cows. Ital J Anim Sci. 2014;13(1):10-4. http://doi.org/10.4081/ijas.2014.2978.
Salihu BZ, Gana AK, Apuyor BO. Castor oil plant (Ricinus communis L.): botany, ecology and uses. Int J Sci Res. 2014;3(5):1333-41. http://doi.org/10.1007/978-1-4020-9827-7.
Scarpa A, Guerci A. Various uses of the castor oil plant (Ricinus communis L.) a review. J Ethnopharmacol. 1982;5(2):117-37. http://doi.org/10.1016/0378-8741(82)90038-1. PMid:7035750.
Schelling GT. Monensin mode of action in the rumen. J Anim Sci. 1984;58(6):1518-27. http://doi.org/10.2527/jas1984.5861518x. PMid:6378867.
Silva LB, Silva W, Macedo MLR, Peres MTLP. Effects of croton urucurana extracts and crude resin on Anagasta kuehniella (Lepidoptera: Pyralidae). Braz Arch Biol Technol. 2009;52(3):653-64. http://doi.org/10.1590/S1516-89132009000300018.
Simionatto E, Bonani VFL, Morel AF, Poppi NR, Raposo Júnior JL, Stuker CZ, Peruzzo GM, Peres MTLP, Hess SC. Chemical composition and evaluation of antibacterial and antioxidant activities of the essential oil of Croton urucurana Baillon (Euphorbiaceae) stem bark. J Braz Chem Soc. 2007;5(18):879-85. http://doi.org/10.1590/S0103-50532007000500002.
Soldera CC, Zanella GN, Frasson APZ. Avaliação da atividade antibacteriana de Croton urucurana. Contexto Saúde. 2010;10(19):25-31.
Tilley JMA, Terry RA. A two-stage technique for the in vitro digestion of forage crops. Grass Forage Sci. 1963;18(2):104- 11. http://doi.org/10.1111/j.1365-2494.1963.tb00335.x.
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