New insights into the distribution, prevalence and pathogenesis of Mycoplasma hyopneumoniae infection in pigs
DOI:
https://doi.org/10.11606/issn.1678-4456.bjvras.2025.233476Keywords:
Swine enzootic pneumonia, Porcine respiratory disease control, Virulence factors, Economic impact, Co-infections in swine pneumoniaAbstract
Swine respiratory diseases significantly impact animal health and welfare, compromising both productive efficiency and the economic outcomes for producers. Among the primary etiological agents, Mycoplasma hyopneumoniae stands out due to its widespread global distribution and high morbidity rates. It predominantly manifests clinically as enzootic pneumonia, leading to reduced performance metrics such as lower weight gain, diminished feed conversion efficiency, and increased susceptibility to co-infections. Understanding the epidemiology and pathogenesis of this bacterium is crucial for developing more effective control strategies to minimize economic losses and enhance herd health. Therefore, this literature review aimed to present studies on the epidemiology of M. hyopneumoniae both globally and in Brazil, as well as to elucidate its pathogenic mechanisms, thereby contributing to a better understanding of the infection and the implementation of more efficient prevention and control measures.
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Almeida HMS, Mechler-Dreibi ML, SonálioK, Ferraz MES, Storino GY, Barbosa FO, Maes D, Montassier HJ, Oliveira LG. Cytokine expression and Mycoplasma hyopneumoniae burden in the development of lung lesions in experimentally inoculated pigs. Vet Microbiol. 2020;244:108647. https://doi.org/10.1016/j.vetmic.2020.108647. PMid:32402328.
Almeida HMS, Sonalio K, Mechler-Dreibi ML, Petri FAM, Storino GY, Maes D, Oliveira LG. Experimental infection with Mycoplasma hyopneumoniae strain 232 in swine influences the lower respiratory microbiota. Vet Sci. 2022;9(12):674. https://doi.org/10.3390/vetsci9120674. PMid:36548835.
Andrade MR, Daniel AGS, Zarate JB, Sato JPH, Santos LF, GuedesRMC. Genetic diversity of Mycoplasma hyopneumoniae in finishing pigs in Minas Gerais. Pesqui Vet Bras. 2023;43:e07155. https://doi.org/10.1590/1678-5150-PVB-7155.
Arruda LP, MalcherC, Petri FAM, da Silva DG, Storino GY, AlmeidaHMS, SonalioK, Toledo LT, Oliveira LG. Pathological analysis and etiological assessment of pulmonary lesions and its association with pleurisy in slaughtered pigs. Vet Microbiol. 2024;292:110039. https://doi.org/10.1016/j.vetmic.2024.110039. PMid:38502977.
Assao VS, Scatamburlo TM, Araujo EN, Santos MR, PereiraCER, Guedes RMC, Bressan GC, Fietto JLR, Chang YF, Moreira MAS, Silva-Júnior A. Genetic variation of Mycoplasma hyopneumoniae from Brazilian field samples. BMC Microbiol. 2019;19(1):234. https://doi.org/10.1186/s12866-019-1603-7. PMid:31660853.
Associação Brasileira de Proteína Animal [Internet]. Relatório anual. São Paulo: ABPA; 2024 [cited 2025 Feb 19]. Available from: https://abpa-br.org/abpa-relatorio-anual/.
Bai F, Ni B, Liu M, Feng Z, Xiong Q, Shao G. Mycoplasma hyopneumoniae-derived lipid-associated membrane proteins induce inflammation and apoptosis in porcine peripheral blood mononuclear cells in vitro. Vet Microbiol. 2015;175(1):58-67. https://doi.org/10.1016/j.vetmic.2014.11.013. PMid:25481242.
Balestrin E, Wolf JM, Wolf LM, Fonseca ASK, Ikuta N, Siqueira FM, Lunge VR. Molecular detection of respiratory coinfections in pig herds with enzootic pneumonia: a survey in Brazil. J Vet Diagn Invest. 2022;34(2):310-3. https://doi.org/10.1177/10406387211069552. PMid:35034523.
Baraldi TG, Cruz NRN, Pereira DA, Galdeano JVB, Gatto IRH, Silva AFD, Panzardi A, Linhares DCL, Mathias LA, Oliveira LG. Antibodies against Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae and influenza virus and their relationships with risk factors, clinical signs and lung lesions in pig farms with one-site production systems in Brazil. Prev Vet Med. 2019;171:104748. https://doi.org/10.1016/j.prevetmed.2019.104748. PMid:31470290.
Betlach AM, Valeris-Chacin R, Singer RS, Allerson M, Pieters M. Natural transmission and detection of Mycoplasma hyopneumoniae in a naïve gilt population. Vet Microbiol. 2020;248:108819. https://doi.org/10.1016/j.vetmic.2020.108819. PMid:32891949.
Boeters M, Garcia-Morante B, van Schaik G, Segalés J, Rushton J, Steeneveld W. The economic impact of endemic respiratory disease in pigs and related interventions - a systematic review. Porcine Health Manag. 2023;9(1):45. https://doi.org/10.1186/s40813-023-00342-w. PMid:37848972.
Calsamiglia M, Collins JE, Pijoan C. Correlation between the presence of enzootic pneumonia lesions and detection of Mycoplasma hyopneumoniae in bronchial swabs by PCR. Vet Microbiol. 2000;76(3):299-303. https://doi.org/10.1016/S0378-1135(00)00245-5. PMid:10973704.
Charlebois A, Marois-Créhan C, Hélie P, Gagnon CA, GottschalkM, ArchambaultM. Genetic diversity of Mycoplasma hyopneumoniae isolates of abattoir pigs. Vet Microbiol. 2014; 168(2-4):348-56. https://doi.org/10.1016/j.vetmic.2013.11.006. PMid:24345410.
Clavijo MJ, Hu D, Krantz S, Cano JP, Maróstica TP, HenaoDiazA, Poeta SilvaAPS, Hemker D, Tapia E, Zimmerman S, Fano E, Polson D, Fitzgerald R, TuckerA, Main R, Wang C, Zimmerman JJ, Rotolo ML. Mycoplasma hyopneumoniae surveillance in pig populations: establishing sampling guidelines for detection in growing Pigs. J Clin Microbiol. 2021;59(5):e03051-20. https://doi.org/10.1128/JCM.03051-20. PMid:33597256.
Čobanović N, Karabasil N, Cojkić A, Vasilev D, Stajković S. Carcass quality and hematological alterations associated with lung lesions in slaughter pigs. Lucr Stiint Zooteh Biotehnol. 2016;49(1):236-40.
Coldebella A, Kich JD, Albuquerque ER, Buosi RJ. Avaliação dos dados de abate e condenações/ desvios de suínos registrados no Sistema de Informações Gerenciais do Serviço de Inspeção Federal nos anos de 2012 a 2014. Concórdia: Embrapa Suínos e Aves; 2018.
Conti ERD, TakeutiKL, SchwertzCI, Bianchi RM, Driemeier D, Barcellos DE. Agents of pneumonia in slaughtered pigs in southern Brazil. Pesq Vet Bras. 2021;41:e06669. https://doi.org/10.1590/1678-5150-pvb-6669.
Dutra MC, Moreno LZ, Dias RA, MorenoAM. Antimicrobial use in brazilian swine herds: assessment of use and reduction examples. Microorganisms. 2021;9(4):881. https://doi.org/10.3390/microorganisms9040881. PMid:33924277.
Fablet C, Marois C, Dorenlor V, Eono F, Eveno E, Jolly JP, Le Devendec L, Kobisch M, Madec F, Rose N. Bacterial pathogens associated with lung lesions in slaughter pigs from 125 herds. Res Vet Sci. 2012;93(2):627-30. https://doi.org/10.1016/j.rvsc.2011.11.002. PMid:22133708.
Fano E, Pijoan C, Dee S, Deen J. Effect of Mycoplasma hyopneumoniae colonization at weaning on disease severity in growing pigs. Can J Vet Res. 2007;71(3):195-200. PMid:17695594.
Ferraz MES, Almeida HMS, Storino GY, Sonálio K, Souza MR, Moura CAA, Costa WMT, Lunardi L, Linhares DCL, Oliveira LG. Lung consolidation caused by Mycoplasma hyopneumoniae has a negative effect on productive performance and economic revenue in finishing pigs. Prev Vet Med. 2020;182:105091. https://doi.org/10.1016/j.prevetmed.2020.105091. PMid:32683190.
Ferreira MM, Mechler-Dreibi ML, Sonalio K, Almeida HMS, Ferraz MES, Jacintho APP, Maes D, Oliveira LG. Co-infections by Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare in macroscopic lesions of lung consolidation of pigs at slaughter. Vet Microbiol. 2021;258:109123. https://doi.org/10.1016/j.vetmic.2021.109123. PMid:34023636.
Galdeano JVB, Baraldi TG, Ferraz MES, De Souza Almeida HM, Mechler-Dreibi ML, Costa WMT, Montassier HJ, Mathias LA, Oliveira LG. Cross-sectional study of seropositivity, lung lesions and associated risk factors of the main pathogens of Porcine Respiratory Diseases Complex (PRDC) in Goiás, Brazil. Porcine Health Manag. 2019;5(1):23. https://doi.org/10.1186/s40813-019-0130-0. PMid:31636919.
Gulliksen SM, Baustad B, Framstad T, Jørgensen A, Skomsøy A, Kjelvik O, Gjestvang M, Grøntvedt CA, Lium B. Successful eradication of Mycoplasma hyopneumoniae from the Norwegian pig population – 10 years later. Porcine Health Manag. 2021;7(1):37. https://doi.org/10.1186/s40813-021-00216-z. PMid:34001280.
Haesebrouck F, Pasmans F, Chiers K, Maes D, Ducatelle R, DecostereA. Efficacy of vaccines against bacterial diseases in swine: what can we expect? Vet Microbiol. 2004;100 (3-4):255-68. https://doi.org/10.1016/j.vetmic.2004.03.002. PMid:15145504.
Kich JD, PontesAP. Análise atual das doenças respiratórias no Brasil. In: Congresso Brasileiro de Veterinários Especialistas em Suínos; 2001; Porto Alegre. Porto Alegre: Associação Brasileira de Veterinários Especialistas em Suínos; 2001. p. 58-67.
Leal FMA, Virginio VG, Martello CL, Paes JA, Borges TJ, Jaeger N, Bonorino C, Ferreira HB. Mycoplasma hyopneumoniae and Mycoplasma flocculare differential domains from orthologous surface proteins induce distinct cellular immune responses in mice. Vet Microbiol. 2016;190:50-7. https://doi.org/10.1016/j.vetmic.2016.05.008. PMid:27283856.
Lima AC, Braga ER, Panneitz AK, Petri FA, Castro JH, Dias RG, de Oliveira LG. Detection of anti-Mycoplasma hyopneumoniae antibodies in backyard pigs in the state of Paraná, Brazil. J Vet Diagn Invest. 2024;36(6):907-9. https://doi.org/10.1177/10406387241265986. PMid:39175302.
Lorenzo H, Quesada Ó, Assunçao P, Castro A, Rodríguez F. Cytokine expression in porcine lungs experimentally infected with Mycoplasma hyopneumoniae. Vet Immunol Immunopathol. 2006;109(3-4):199-207. https://doi.org/10.1016/j.vetimm.2005.07.021. PMid:16325264.
Maes D, Sibila M, Kuhnert P, Segalés J, Haesebrouck F, Pieters M. Update on Mycoplasma hyopneumoniae infections in pigs: knowledge gaps for improved disease control. Transbound Emerg Dis. 2018;65(Suppl 1):110-24. https://doi.org/10.1111/tbed.12677. PMid:28834294.
Maes D, Sibila M, Pieters M, Haesebrouck F, Segalés J, Oliveira LG. Review on the methodology to assess respiratory tract lesions in pigs and their production impact. Vet Res. 2023;54(1):8. https://doi.org/10.1186/s13567-023-01136-2. PMid:36726112.
Malcher CS, Petri FAM, Arruda LP, de Aguiar GA, Storino GY, Sonalio K, Toledo LT, Hirose F, Oliveira LG. Health– economic impact attributable to occurrence of pleurisy and pneumonia lesions in finishing pigs. Vet Sci. 2024;11(12):668. https://doi.org/10.3390/vetsci11120668. PMid:39729008.
Marois C, Dory D, Fablet C, Madec F, Kobisch M. Development of a quantitative Real-Time TaqMan PCR assay for determination of the minimal dose of Mycoplasma hyopneumoniae strain 116 required to induce pneumonia in SPF pigs. J Appl Microbiol. 2010;108(5):1523-33. https://doi.org/10.1111/j.1365-2672.2009.04556.x. PMid:19811567.
Martelli P, Saleri R, Cavalli V, De Angelis E, Ferrari L, Benetti M, Ferrarini G, Merialdi G, Borghetti P. Systemic and local immune response in pigs intradermally and intramuscularly injected with inactivated Mycoplasma hyopneumoniae vaccines. Vet Microbiol. 2014;168 (2-4):357-64. https://doi.org/10.1016/j.vetmic.2013.11.025. PMid:24332702.
Merialdi G, Dottori M, Bonilauri P, Luppi A, Gozio S, Pozzi P, Spaggiari B, Martelli P. Survey of pleuritis and pulmonary lesions in pigs at abattoir with a focus on the extent of the condition and herd risk factors. Vet J. 2012;193(1):234-9. https://doi.org/10.1016/j.tvjl.2011.11.009. PMid:22182431.
MeynsT, MaesD, CalusD, Ribbens S, DewulfJ, ChiersK, Kruif A, Cox E, DecostereA, Haesebrouck F. Interactions of highly and low virulent Mycoplasma hyopneumoniae isolates with the respiratory tract of pigs. Vet Microbiol. 2007;120(1-2):87-95. https://doi.org/10.1016/j.vetmic.2006.10.010. PMid:17123752.
Morés MAZ, Oliveira Filho JX, Rebelatto R, Klein CS, Barcellos DEN, ColdebellaA, Morés N. Aspectos patológicos e microbiológicos das doenças respiratórias em suínos de terminação no Brasil. Pesq Vet Bras. 2015;35(8):725-33. https://doi.org/10.1590/S0100-736X2015000800004.
Opriessnig T, Giménez-Lirola LG, Halbur PG. Polymicrobial respiratory disease in pigs. Anim Health Res Rev. 2011;12(2):133-48. https://doi.org/10.1017/S1466252311000120. PMid:22152290.
Pallarés FJ, Añón J, Rodríguez-Gómez I, Gómez-Laguna J, Fabré R, Sánchez-Carvajal J, Ruedas-Torres I, Carrasco L. Prevalence of mycoplasma-like lung lesions in pigs from commercial farms from Spain and Portugal. Porcine Health Manag. 2021;7(1):26. https://doi.org/10.1186/s40813-021-00204-3. PMid:33685489.
PanneitzAK, BragaER, PetriFAM, MenegattJCO, Driemeier D, Maes D, Oliveira LG. Exploring the genetic diversity of Mycoplasma hyopneumoniae in pigs with pneumonia and pleurisy at slaughter. Microorganisms. 2024;12(10):1988. https://doi.org/10.3390/microorganisms12101988. PMid:39458297.
Petri FAM, Ferreira GC, Arruda LP, Malcher CS, Storino GY, Almeida HMS, Sonalio K, Silva DG, de Oliveira LG. Associations between pleurisy and the main bacterial pathogens of the Porcine Respiratory Diseases Complex (PRDC).Animals. 2023;13(9):1493. https://doi.org/10.3390/ani13091493. PMid:37174529.
Pieters M, Maes D. Mycoplasmosis. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW, Zhang J, editors. Diseases of Swine. Hoboken: John Wiley & Sons; 2019. p. 863-83. https://doi.org/10.1002/9781119350927.ch56.
Pieters M, Pijoan C, Fano E, Dee S. An assessment of the duration of Mycoplasma hyopneumoniae infection in an experimentally infected population of pigs. Vet Microbiol. 2009;134(3-4):261-6. https://doi.org/10.1016/j.vetmic.2008.08.016. PMid:18835112.
Przyborowska P, Lewko-Wojtowicz R, Cybulski P, Maes D, Tobolski D. Impact of porcine respiratory disease complex on carcass weight and meatiness: quantitative insights from a mixed-model analysis. BMC Vet Res. 2024;20(1):554. https://doi.org/10.1186/s12917-024-04410-3. PMid:39643874.
Rautiainen E, Oravainen J, Virolainen JV, Tuovinen V. Regional eradication of Mycoplasma hyopneumoniae from pig herds and documentation of freedom of the disease.Acta Vet Scand. 2001;42(3):355-64. https://doi.org/10.1186/1751-0147-42-355. PMid:11887396.
Razin S, Jacobs E. Mycoplasma adhesion. J Gen Microbiol. 1992;138(3):407-22. https://doi.org/10.1099/00221287-138-3-407. PMid:1593256.
Reis R, LemosJ, Cavalcante J. Estudo das lesöes pulmonares de suínos de abate.Arq Bras Med Vet Zootec. 1992;44(5):407-18.
Rodríguez F, Batista M, Hernández JN, AfonsoAM, Poveda JB. Relationship between expression of interleukin-5 and interleukin-13 by epithelial cells and bronchiolar changes in pigs infected with Mycoplasma hyopneumoniae.J Comp Pathol. 2016;154(2-3):165-8. https://doi.org/10.1016/j.jcpa.2016.01.007. PMid:26922858.
Santos LF, Sreevatsan S, Torremorell M, Moreira MAS, Sibila M, Pieters M. Genotype distribution of Mycoplasma hyopneumoniae in swine herds from different geographical regions. Vet Microbiol. 2015;175(2-4):374-81. https://doi.org/10.1016/j.vetmic.2014.11.018. PMid:25497236.
Sibila M, Pieters M, Molitor T, Maes D, Haesebrouck F, Segalés J. Current perspectives on the diagnosis and epidemiology of Mycoplasma hyopneumoniae infection. Vet J. 2009;181(3):221-31. https://doi.org/10.1016/j.tvjl.2008.02.020. PMid:18396428.
Silva GS, Yeske P, Morrison RB, Linhares DCL. Benefit-cost analysis to estimate the payback time and the economic value of two Mycoplasma hyopneumoniae elimination methods in breeding herds. Prev Vet Med. 2019;168:95-102. https://doi.org/10.1016/j.prevetmed.2019.04.008. PMid:31097130.
Silva APS, Marostica TP, McDaniel A, Arruda BL, Alonso C, Derscheid R, Yeske P, Linhares DCL, Giménez-Lirola L, Karriker L, Fano E, Zimmerman JJ, Clavijo MJ. Comparison of Mycoplasma hyopneumoniae response to infection by route of exposure. Vet Microbiol. 2021;258:109118. https://doi.org/10.1016/j.vetmic.2021.109118. PMid:34058523.
Sobestiansky J, Barcellos D. Doenças dos suínos. 3. ed. Goiânia: Cânone; 2022. Sobestiansky J, Costa OAD, Morés N, Barioni WJ, PifferIA, Guzzo R. Estudos ecopatológicos das doenças respiratórias dos suínos: prevalência e impacto econômico em sistemas de produção dos estados de Santa Catarina, Rio Grande do Sul e Paraná. Concórdia: Embrapa Suínos e Aves; 2001.
Sonalio K, Almeida HMS, Mechler-Dreibi ML, Storino GY, Haesebrouck F, Maes D, Oliveira LG. Influence of Mycoplasma hyopneumoniae natural infection on the respiratory microbiome diversity of finishing pigs. Vet Res. 2022;53(1):20. https://doi.org/10.1186/s13567-022-01038-9. PMid:35303928.
Sørensen V, Ahrens P, Barfod K, Feenstra AA, Feld NC, Friis NF, Bille-Hansen V, Jensen NE, Pedersen MW. Mycoplasma hyopneumoniae infection in pigs: duration of the disease and evaluation of four diagnostic assays. Vet Microbiol. 1997;54(1):23-34. https://doi.org/10.1016/S0378-1135(96)01266-7. PMid:9050168.
Sosa C, Blois A, Ibáñez F, Tamiozzo P. Genetic diversity of Mycoplasma hyopneumoniae in Mendoza province. Rev Argent Microbiol. 2019;51(3):229-33. https://doi.org/10.1016/j.ram.2018.07.004. PMid:30651187.
Stärk KDC, Miserez R, Siegmann S, Ochs H, Infanger P, Schmidt J. A successful national control programme for enzootic respiratory diseases in pigs in Switzerland. Rev Sci Tech. 2007;26(3):595-606. https://doi.org/10.20506/rst.26.3.1768. PMid:18293608.
TakeutiKL, Barcellos DESN, AndradeCP, Almeida LL, Pieters M. Infection dynamics and genetic variability of Mycoplasma hyopneumoniae in self-replacement gilts. Vet Microbiol. 2017;208:18-24. https://doi.org/10.1016/j.vetmic.2017.07.007. PMid:28888635.
Toledo LT, Souza LFL, PereiraCER, Polveiro RC, Bressan GC, Yamatogi RS, JeongKC, Marks FS, DiamantinoCA, Carvalho VHR, Malcher CS, Petri FAM, Oliveira LG, Moreira MAS, Silva-Júnior A. A genetic and virulence characterization of Brazilian strains of Mycoplasma hyopneumoniae. Front Microbiol. 2023;14:1280588. https://doi.org/10.3389/fmicb.2023.1280588. PMid:38075868.
Vangroenweghe FACJ, Labarque GG, Piepers S, StrutzbergMinderK, Maes D. Mycoplasma hyopneumoniae infections in peri-weaned and post-weaned pigs in Belgium and The Netherlands: prevalence and associations with climatic conditions. Vet J. 2015;205(1):93-7. https://doi.org/10.1016/j.tvjl.2015.03.028. PMid:25981930.
Vicca J, Stakenborg T, Maes D, Butaye P, PeetersJ, De Kruif A, Haesebrouck F. Evaluation of virulence of Mycoplasma hyopneumoniae field isolates. Vet Microbiol. 2003;97 (3-4):177-90. https://doi.org/10.1016/j.vetmic.2003.08.008. PMid:14654289.
Vicente AF, Catto D, Allendorf SD, Garcia KCOD, Antunes JMAP, Appolinario CM, Peres MG, Megid J. Soropositividade para Mycoplasma hyopneumoniae em suínos abatidos em frigoríficos daregião central do estado de São Paulo.Arq Bras Med Vet Zootec. 2013;65(6):1899-903. https://doi.org/10.1590/S0102-09352013000600045.
Villarreal I, Meyns T, Dewulf J, Vranckx K, Calus D, Pasmans F, Haesebrouck F, Maes D. The effect of vaccination on the transmission of Mycoplasma hyopneumoniae in pigs under field conditions. Vet J. 2011;188(1):48-52. https://doi.org/10.1016/j.tvjl.2010.04.024. PMid:20605734.
Vranckx K, Maes D, Sacristán RDP, Pasmans F, Haesebrouck F. A longitudinal study of the diversity and dynamics of Mycoplasma hyopneumoniae infections in pig herds. Vet Microbiol. 2012;156(3-4):315-21. https://doi.org/10.1016/j.vetmic.2011.11.007. PMid:22138620.
Wang Q, Cai R, HuangA, WangX, QuW, Shi L, LiC, Yan H. Comparison of oropharyngeal microbiota in healthy piglets and piglets with respiratory disease.Front Microbiol. 2018;9:3218. https://doi.org/10.3389/fmicb.2018.03218. PMid:30627125.
Woolley LK, Fell S, Gonsalves JR, Walker MJ, Djordjevic SP, JenkinsC, EamensGJ. Evaluation of clinical, histological and immunological changes and qPCR detection of Mycoplasma hyopneumoniae in tissues during the early stages of mycoplasmal pneumonia in pigs after experimental challenge with two field isolates. Vet Microbiol. 2012;161(1-2):186-95. https://doi.org/10.1016/j.vetmic.2012.07.025. PMid:22863144.
Yeske P, Valeris-Chacin R, Singer RS, Pieters M. Survival analysis of two Mycoplasma hyopneumoniae eradication methods. Prev Vet Med. 2020;174:104811. https://doi.org/10.1016/j.prevetmed.2019.104811. PMid:31710945.
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