Detection of rabies virus antigen by direct immunoperoxidase and fluorescent antibody test in experimentally inoculated mice, sacrificed in assymptomatic and agonizing state.

Authors

  • Jane Megid Universidade Estadual de Londrina, Londrina, PR
  • Fumio Honma Ito Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.0000-0000.272187-192

Keywords:

Rabies diagnosis, Fluorescent antibody technique, Imunological reactions, intnunoperoxidase, Mice

Abstract

The positivity of the direct immunoperoxidase and the fluorescent antibody test for rabies virus antigen was comparatively evaluated in sixty young mice inoculated intracerebrally with street rabies virus. Thirty mice were sacrificed in assymptomatic state and the others in their agonizing state. The positive results obtained from impression brain smears of assymptomatic animals by direct immunoperoxidase and fluorescent antibody test were 83.3% and 86.6%, respectively. The results found in impression brain smears of agonizing animals revelead 100% positivity in both techniques. The direct immunoperoxidase test presented a relative sensitivity comparable to the
fluorescent antibody test for the detection of rabies virus antigen.

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Published

1990-12-02

Issue

Vol. 27 No. 2 (1990)

Section

PREVENTIVE VETERINARY MEDICINE

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

How to Cite

1.
Megid J, Ito FH. Detection of rabies virus antigen by direct immunoperoxidase and fluorescent antibody test in experimentally inoculated mice, sacrificed in assymptomatic and agonizing state. Braz. J. Vet. Res. Anim. Sci. [Internet]. 1990 Dec. 2 [cited 2024 Nov. 21];27(2):187-92. Available from: https://revistas.usp.br/bjvras/article/view/51851