Comparison between linear smear concentration and cytocentrifugation techniques to evaluate equine bronchoalveolar lavage cytology

Authors

  • Vanessa Viscardi Polícia Militar do Estado do Rio de Janeiro, RJ
  • Joana de Castro Faria Belling
  • Aline Del Carmen Garcia Lopes
  • Gisela Vasconcelos Gioia Universidade Federal Fluminense, Faculdade de Veterinária, Niterói, RJ
  • Rodolpho de Almeida Torres Filho Universidade Federal Fluminense, Faculdade de Veterinária, Niterói, RJ
  • Nayro Xavier de Alencar Universidade Federal Fluminense, Faculdade de Veterinária, Niterói, RJ
  • Daniel Augusto Barroso Lessa Universidade Federal Fluminense, Faculdade de Veterinária, Niterói, RJ

DOI:

https://doi.org/10.11606/issn.2318-3659.v50i1p68-70

Keywords:

Horse, Bronchoalveolar lavage, Cytocentrifugation, Linear smear

Abstract

The bronchoalveolar lavage (BAL) is a sensitive method to diagnose diseases of the distal portion of the lower respiratory tract and has been broadly used by numerous researchers. Cytocentrifugation is the choice cytological preparation technique, but demands specific and costly equipment. Therefore, the present paper intends to verify the applicability of the linear smear technique to evaluate BAL samples. For this, BAL samples of 30 equines were used and the cytological preparations were done by cytocentrifugation and linear smear techniques. All glass microscope slides were fixed and stained with Giemsa for the differential cell count. Regarding the effect of the preparation technique on differential counts, no significant difference in any cell type was found. The linear smear is a reliable alternative and can be recommended as a substitution to cytocentrifugation.

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Published

2013-02-22

Issue

Vol. 50 No. 1 (2013)

Section

PRELIMINARY NOTE

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

How to Cite

1.
Viscardi V, Belling J de CF, Lopes ADCG, Gioia GV, Torres Filho R de A, Alencar NX de, et al. Comparison between linear smear concentration and cytocentrifugation techniques to evaluate equine bronchoalveolar lavage cytology. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2013 Feb. 22 [cited 2025 Apr. 1];50(1):68-70. Available from: https://revistas.usp.br/bjvras/article/view/55840