SDS-PAGE seminal plasma proteins pattern and its correlation with gir breed semen freezability

Authors

  • Marcelo Roncoletta Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Jaboticabal, SP
  • Paulo Henrique Franceschini Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Jaboticabal, SP
  • Vera Fernanda Martins Hossepian de Lima Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Jaboticabal, SP
  • Lúcia Helena Rodrigues Lagoa da Serra Inseminação Artificial, Sertãozinho, SP
  • Marcelo Almeida Oliveira Lagoa da Serra Inseminação Artificial, Sertãozinho, SP
  • Carlos da Silva Lagoa da Serra Inseminação Artificial, Sertãozinho, SP

DOI:

https://doi.org/10.1590/S1413-95961999000200005

Keywords:

Seminal plasma, Cattle, Electrophoresis, Freezing, Semen

Abstract

This study was undertaken to determine whether bovine seminal plasma contained protein markers associated with semen freezability. Seminal plasma was obtained from ten Gir bulls of known fertility and freezability, set into two groups of High (up to 80% of viable sperm after thawing) and Low freezability (less than 50%). SDS-PAGE of seminal plasma samples indicated that one protein (Mr =-20.3;-MW-=-61,800Da) predominated in high-freezability semen. These findings indicate that bull seminal plasma contains freezability-associated proteins which can be used as markers for predicting semen freezability.

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Published

1999-01-01

Issue

Vol. 36 No. 2 (1999)

Section

VETERINARY MEDICINE

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

How to Cite

1.
Roncoletta M, Franceschini PH, Lima VFMH de, Rodrigues LH, Oliveira MA, Silva C da. SDS-PAGE seminal plasma proteins pattern and its correlation with gir breed semen freezability. Braz. J. Vet. Res. Anim. Sci. [Internet]. 1999 Jan. 1 [cited 2024 Nov. 24];36(2):82-6. Available from: https://revistas.usp.br/bjvras/article/view/5766