Long term changes in the culture medium composition and cellular biochemistry of Phacodactylum tricomutum (Bohlin), in batch cultures

Authors

  • Elizabeth Aidar Universidade de São Paulo; Instituto Oceanográfico
  • Roberto Ehrlich Universidade de São Paulo; Instituto Oceanográfico
  • Cristina Sayuri Asano Universidade de São Paulo; Instituto Oceanográfico
  • Teresa Cristina Siqueira Sigaud Universidade de São Paulo; Instituto Oceanográfico

DOI:

https://doi.org/10.1590/S1679-87591991000200004

Keywords:

Phaeodactylum tricornutum, Algae culture, Chlorophylls, Proteins, Nutrients (mineral), Carbohydrates

Abstract

P. tricornutum was grown in batch cultures under 21ºC temperature and continuous irradiance of 183 µE.m-2 . s-1, provided by fluorescent light tubes. Cultures growth were followed by cell countings, "in vivo" fluorescence and cellular protein, carbohydrate and chlorophyll-α content. The maximum value of protein/carbohydrate ratio was found at the exponential phase and the minimum value at the stationary phase. This ratio characterizes the physiological stage of the culture. Cell uptake rates for nitrate, ammonium and phosphate from the media were higher during the lag-phase. Nitrite excretion by the cells were detected following nitrate uptake.

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Published

1991-01-01

Issue

Vol. 39 No. 2 (1991)

Section

Artigos

How to Cite

Long term changes in the culture medium composition and cellular biochemistry of Phacodactylum tricomutum (Bohlin), in batch cultures . (1991). Boletim Do Instituto Oceanografico, 39(2), 131-139. https://doi.org/10.1590/S1679-87591991000200004